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Plasmid
pET6HIS SUMO PINK1 tribolium 145-570
Parent Plasmid
pET15b 6His SUMO1
Expressed
His-SUMO-TcPINK1 145-570
DU Number
DU43656
Genbank
XP_968367
Tag
6His
SUMO
Species
Tribolium castaneum
Sequence of Insert
View Sequence
TACGATATCGACGAAAGCCGCTTTGAATCTAACCCGATCACCCTGAATGA
TCTGAGTCTGGGTAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACT
CCGCAAAAGTCAAAGATGACGAAACCGATGACAATAAATATCCGTTTGCC
CTGAAAATGATGTTCAACTACGATATTCAGAGCAATTCTATGGAAATCCT
GAAAGCTATGTATCGTGAAACCGTTCCGGCGCGCATGTATTACTCAAACC
ACGACCTGAACAATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTG
CCGCCGCACCCGAATATTGTGGCAATCTTTAGCGTTTTCACCGATCTGAT
CCAAGAACTGGAAGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGC
GTCTGCATCCGGAAGGTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATG
AAACGTTATGATTGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTAC
CCGCACCTCCCTGCTGCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATA
TGACGGCCCACGGTATTGCACATCGTGATCTGAAATCAGACAACCTGCTG
CTGGATACCTCAGAACCGGAATCGCCGATTCTGGTTATCTCGGATTTTGG
CTGCTGTCTGGCGGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTT
ACGAAATGGATAAAGGCGGTAATACGGCGCTGATGGCCCCGGAAATTATC
TGCCAGAAACCGGGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCT
GTGGGCGGTGGGTGCAATCGCTTACGAAATCTTCAACTGTCACAATCCGT
TCTACGGCCCGTCTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTG
CCGAAACTGCCGGACGAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAA
CCTGCTGAAACGTAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCA
ATGTCTGCCAGCTGTTTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGT
CTGAAAGTTCCGACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGAC
CACGAAAGTCCTGTGCGAAGGCAAAATCAACAACAAATCCTTCGGTGAAA
AATTCACCCGTAATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGT
TCCTTCCTGTGTCGCGCGAAACTGGCTAATGTTCGTAATGCTCTGCACTG
GATTCAAGAAAACCTGCCGGAACTGGATTAAGCGGCCGC
TCTGAGTCTGGGTAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACT
CCGCAAAAGTCAAAGATGACGAAACCGATGACAATAAATATCCGTTTGCC
CTGAAAATGATGTTCAACTACGATATTCAGAGCAATTCTATGGAAATCCT
GAAAGCTATGTATCGTGAAACCGTTCCGGCGCGCATGTATTACTCAAACC
ACGACCTGAACAATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTG
CCGCCGCACCCGAATATTGTGGCAATCTTTAGCGTTTTCACCGATCTGAT
CCAAGAACTGGAAGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGC
GTCTGCATCCGGAAGGTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATG
AAACGTTATGATTGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTAC
CCGCACCTCCCTGCTGCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATA
TGACGGCCCACGGTATTGCACATCGTGATCTGAAATCAGACAACCTGCTG
CTGGATACCTCAGAACCGGAATCGCCGATTCTGGTTATCTCGGATTTTGG
CTGCTGTCTGGCGGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTT
ACGAAATGGATAAAGGCGGTAATACGGCGCTGATGGCCCCGGAAATTATC
TGCCAGAAACCGGGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCT
GTGGGCGGTGGGTGCAATCGCTTACGAAATCTTCAACTGTCACAATCCGT
TCTACGGCCCGTCTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTG
CCGAAACTGCCGGACGAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAA
CCTGCTGAAACGTAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCA
ATGTCTGCCAGCTGTTTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGT
CTGAAAGTTCCGACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGAC
CACGAAAGTCCTGTGCGAAGGCAAAATCAACAACAAATCCTTCGGTGAAA
AATTCACCCGTAATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGT
TCCTTCCTGTGTCGCGCGAAACTGGCTAATGTTCGTAATGCTCTGCACTG
GATTCAAGAAAACCTGCCGGAACTGGATTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGYDIDESRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNK
YPFALKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANR
RKHLPPHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSL
FLLMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKS
DNLLLDTSEPESPILVISDFGCCLADKTNGLSLPYTSYEMDKGGNTALMA
PEIICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYK
EGDLPKLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTW
LKPGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEY
LLISSFLCRAKLANVRNALHWIQENLPELD*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGYDIDESRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNK
YPFALKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANR
RKHLPPHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSL
FLLMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKS
DNLLLDTSEPESPILVISDFGCCLADKTNGLSLPYTSYEMDKGGNTALMA
PEIICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYK
EGDLPKLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTW
LKPGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEY
LLISSFLCRAKLANVRNALHWIQENLPELD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
in pET - please make wild-type and kinase-inactive versions (D359A)
100042 R 1000 1001 1047 19007
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