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Synonyms
BRPK, FLJ27236, PARK6
Plasmid
pcDNA5frtTO HSP60 1-27 + PINK1 104-513 + C-FLAG
Parent Plasmid
pcDNA5 FRT/TO
Expressed
HSP60 (1-27) - PINK1 (104-513) -FLAG
DU Number
DU44361
Genbank
NM_032409.2
Tag
FLAG
Species
Human
Sequence of Insert
View Sequence
AAGCTTGCCACCATGCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACC
GGTGTCCAGGGTACTGGCTCCTCATCTCACTCGGGCTTATGCCGGATCCA
TGTTCGGGCTCGGGCTGGGCCTCATCGAGGAAAAACAGGCGGAGAGCCGG
CGGGCGGTCTCGGCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAAAAG
CAAGCCGGGGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTCGGC
TGGAGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCT
GTGTATGAAGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGACAAA
GAGCACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAG
AAGGGCAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAG
ATGATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACAC
AATGAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGT
ATGGAGCAGTCACTTACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCC
CCTCACCCCAACATCATCCGGGTTCTCCGCGCCTTCACCTCTTCCGTGCC
GCTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCC
TCCACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAG
AACTATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCC
CCGCCTCGCCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATC
TGGTTCAACAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCTT
GTGGAGCTGGACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGATTTTGG
CTGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCAGCT
GGTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCC
ACGGCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGC
CTGGGCAGTGGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCT
TCTACGGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAGGCT
CAGCTACCTGCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTTGGT
GAGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAGTAG
CCGCAAATGTGCTTCATCTAAGCCTCTGGGGTGACTACAAGGACGATGAC
GATAAGTGAGCGGCCGC
GGTGTCCAGGGTACTGGCTCCTCATCTCACTCGGGCTTATGCCGGATCCA
TGTTCGGGCTCGGGCTGGGCCTCATCGAGGAAAAACAGGCGGAGAGCCGG
CGGGCGGTCTCGGCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAAAAG
CAAGCCGGGGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTCGGC
TGGAGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCT
GTGTATGAAGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGACAAA
GAGCACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAG
AAGGGCAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAG
ATGATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACAC
AATGAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGT
ATGGAGCAGTCACTTACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCC
CCTCACCCCAACATCATCCGGGTTCTCCGCGCCTTCACCTCTTCCGTGCC
GCTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCC
TCCACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAG
AACTATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCC
CCGCCTCGCCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATC
TGGTTCAACAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCTT
GTGGAGCTGGACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGATTTTGG
CTGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCAGCT
GGTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCC
ACGGCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGC
CTGGGCAGTGGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCT
TCTACGGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAGGCT
CAGCTACCTGCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTTGGT
GAGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAGTAG
CCGCAAATGTGCTTCATCTAAGCCTCTGGGGTGACTACAAGGACGATGAC
GATAAGTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MLRLPTVFRQMRPVSRVLAPHLTRAYAGSMFGLGLGLIEEKQAESRRAVS
ACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSAAVYEA
TMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIKMMWN
ISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPN
IIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPC
TLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELD
PDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARP
GPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPA
LPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGDYKDDDDK*
ACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSAAVYEA
TMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIKMMWN
ISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPN
IIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPC
TLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELD
PDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARP
GPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPA
LPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGDYKDDDDK*
Vector Type
Mammalian
Antibiotic
Amp
Comments
HSP60 1-27 at N-terminus and C-FLAG. HSP60 1-27 may be swapped out HindIII/BamHI and the PINK1-FLAG using BamHI/NotI.
100042 R 1000 1001 1047 19007
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