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Plasmid
pET6HIS SUMO PINk1 Tribolium 158-570
Parent Plasmid
pET15b 6His SUMO1
Expressed
His-SUMO TcPINK1 (158-570)
DU Number
DU39868
Genbank
XP_968367.1
Tag
SUMO
Species
Tribolium castaneum
Sequence of Insert
View Sequence
GGAACCCTGAATGATCTGAGTCTGGGTAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAGATGACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATGTTCAACTACGATATTCAGAGCAATTCTATGGAAATCCTGAAAGCTATGTATCGTGAAACCGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACAATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAATATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGAAGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAGGTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGATTGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCTGCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACGGTATTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAACCGGAATCGCCGATTCTGGTTATCTCGGATTTTGGCTGCTGTCTGGCGGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAGGCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCGGGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGCAATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGTCTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTGCCGAAACTGCCGGACGAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAACGTAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGTTTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCGACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTGCGAAGGCAAAATCAACAACAAATCCTTCGGTGAAAAATTCACCCGTAATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGCGCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAACCTGCCGGAACTGGATTAAgcggccgc
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGTLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLPPHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLDTSEPESPILVISDFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPKLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLISSFLCRAKLANVRNALHWIQENLPELD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
With His-SUMO tag with no overhang, plus kinase dead (D359A)
100042 R 1000 1001 1047 19007
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