LRRK2

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Plasmid
pGEX-LRRK2-Kin Dom
Parent Plasmid
pGEX6P1
Expressed
GST-LRRK2-Kin Dom
DU Number
DU2985
Genbank
AY792511
Tag
GST
Species
Human
Sequence of Insert
GGATCCGCCCCTGACTTGATTTTGGCTGACCTGCCTAGAAATATTATGTT
GAATAGTGATGAGTTGGAATTTGAACAAGCTCCAGAGTTTCTCCTAGGTG
ATGGCAGTTTTGGATCAGTTTACCGAGCAGCCTATGAAGGAGAAGAAGTG
GCTGTGAAGATTTTTAATAAACATACATCACTCAGGCTGTTAAGACAAGA
GCTTGTGGTGCTTTGCCACCTCCACCACCCCAGTTTGATATCTTTGCTGG
CAGCTGGGATTCGTCCCCGGATGTTGGTGATGGAGTTAGCCTCCAAGGGT
TCCTTGGATCGCCTGCTTCAGCAGGACAAAGCCAGCCTCACTAGAACCCT
ACAGCACAGGATCGCACTCCACGTAGCTGATGGTTTGAGATACCTCCACT
CAGCCATGATTATATACCGAGACCTGAAACCCCACAATGTGCTGCTTTTC
ACACTGTATCCCAATGCTGCCATCATCGCAAAGATTGCTGACTACGGCAT
TGCTCAGTACTGCTGTAGAATGGGGATAAAAACATCAGAGGGCACACCAG
GGTTTCGTGCACCTGAAGTTGCCAGAGGAAATGTCATTTATAACCAACAG
GCTGATGTTTATTCATTTGGTTTACTACTCTATGACATTTTGACAACTGG
AGGTAGAATAGTAGAGGGTTTGAAGTTTCCAAATGAGTTTGATGAATTAG
AAATACAAGGAAAATTACCTGATCCAGTTAAAGAATATGGTTGTGCCCCA
TGGCCTATGGTTGAAAGATTAATTAAACAGTGTTTGAAAGAAAATCCTCA
AGAAAGGCCTACTTCTGCCCAGGTCTTTGACATTTTGAATTCAGCTGAAT
TAGTCTGTCTGACGAGACGCATTTTATAGGATCC
Amino Acid Sequence
MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSAPDLILADLPRNI
MLNSDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLR
QELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTR
TLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADY
GIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILT
TGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVERLIKQCLKEN
PQERPTSAQVFDILNSAELVCLTRRIL*
Vector Type
Bacterial
Antibiotic
Amp
Comments
N terminal GST for mammalian expression containing catalytic domain of LRRK2. Differs to genbank at N1827S K2064R. Mutations also in DKFZp451G151 EST.
All LRRK2 plasmids MUST be grown at 30 degrees C or less to prevent recombination. Contains SNP S1647T
100042 R 1000 1001 1047 19007
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