LRRK2

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Plasmid
pFastbac GST LRRK2 H970-E2527(end) G2019S
Parent Plasmid
pFastbac GST
Expressed
GST-LRRK2 970-end G2019S
DU Number
DU17285
Genbank
NM_198578.3 GI:171846277 with silent changes c4872a, a4911g, and coding change t4939a (S1647T) (present in other DU LRRK2 clones)
Tag
GST
Species
Human
Sequence of Insert
GGATCCCATTCAGACAGCATTTCTTCTCTGGCTTCTGAGAGAGAATATAT
TACATCACTAGACCTTTCAGCAAATGAACTAAGAGATATTGATGCCCTAA
GCCAGAAATGCTGTATAAGTGTTCATTTGGAGCATCTTGAAAAGCTGGAG
CTTCACCAGAATGCACTCACGAGCTTTCCACAACAGCTATGTGAAACTCT
GAAGAGTTTGACACATTTGGACTTGCACAGTAATAAATTTACATCATTTC
CTTCTTATTTGTTGAAAATGAGTTGTATTGCTAATCTTGATGTCTCTCGA
AATGACATTGGACCCTCAGTGGTTTTAGATCCTACAGTGAAATGTCCAAC
TCTGAAACAGTTTAACCTGTCATATAACCAGCTGTCTTTTGTACCTGAGA
ACCTCACTGATGTGGTAGAGAAACTGGAGCAGCTCATTTTAGAAGGAAAT
AAAATATCAGGGATATGCTCCCCCTTGAGACTGAAGGAACTGAAGATTTT
AAACCTTAGTAAGAACCACATTTCATCCCTATCAGAGAACTTTCTTGAGG
CTTGTCCTAAAGTGGAGAGTTTCAGTGCCAGAATGAATTTTCTTGCTGCT
ATGCCTTTCTTGCCTCCTTCTATGACAATCCTAAAATTATCTCAGAACAA
ATTTTCCTGTATTCCAGAAGCAATTTTAAATCTTCCACACTTGCGGTCTT
TAGATATGAGCAGCAATGATATTCAGTACCTACCAGGTCCCGCACACTGG
AAATCTTTGAACTTAAGGGAACTCTTATTTAGCCATAATCAGATCAGCAT
CTTGGACTTGAGTGAAAAAGCATATTTATGGTCTAGAGTAGAGAAACTGC
ATCTTTCTCACAATAAACTGAAAGAGATTCCTCCTGAGATTGGCTGTCTT
GAAAATCTGACATCTCTGGATGTCAGTTACAACTTGGAACTAAGATCCTT
TCCCAATGAAATGGGGAAATTAAGCAAAATATGGGATCTTCCTTTGGATG
AACTGCATCTTAACTTTGATTTTAAACATATAGGATGTAAAGCCAAAGAC
ATCATAAGGTTTCTTCAACAGCGATTAAAAAAGGCTGTGCCTTATAACCG
AATGAAACTTATGATTGTGGGAAATACTGGGAGTGGTAAAACCACCTTAT
TGCAGCAATTAATGAAAACCAAGAAATCAGATCTTGGAATGCAAAGTGCC
ACAGTTGGCATAGATGTGAAAGACTGGCCTATCCAAATAAGAGACAAAAG
AAAGAGAGATCTCGTCCTAAATGTGTGGGATTTTGCAGGTCGTGAGGAAT
TCTATAGTACTCATCCCCATTTTATGACGCAGCGAGCATTGTACCTTGCT
GTCTATGACCTCAGCAAGGGACAGGCTGAAGTTGATGCCATGAAGCCTTG
GCTCTTCAATATAAAGGCTCGCGCTTCTTCTTCCCCTGTGATTCTCGTTG
GCACACATTTGGATGTTTCTGATGAGAAGCAACGCAAAGCCTGCATGAGT
AAAATCACCAAGGAACTCCTGAATAAGCGAGGGTTCCCTGCCATACGAGA
TTACCACTTTGTGAATGCCACCGAGGAATCTGATGCTTTGGCAAAACTTC
GGAAAACCATCATAAACGAGAGCCTTAATTTCAAGATCCGAGATCAGCTT
GTTGTTGGACAGCTGATTCCAGACTGCTATGTAGAACTTGAAAAAATCAT
TTTATCGGAGCGTAAAAATGTGCCAATTGAATTTCCCGTAATTGACCGGA
AACGATTATTACAACTAGTGAGAGAAAATCAGCTGCAGTTAGATGAAAAT
GAGCTTCCTCACGCAGTTCACTTTCTAAATGAATCAGGAGTCCTTCTTCA
TTTTCAAGACCCAGCACTGCAGTTAAGTGACTTGTACTTTGTGGAACCCA
AGTGGCTTTGTAAAATCATGGCACAGATTTTGACAGTGAAAGTGGAAGGT
TGTCCAAAACACCCTAAGGGAATTATTTCGCGTAGAGATGTGGAAAAATT
TCTTTCAAAGAAAAGGAAATTTCCAAAGAACTACATGACACAGTATTTTA
AGCTCCTAGAAAAATTCCAGATTGCTTTGCCAATAGGAGAAGAATATTTG
CTGGTTCCAAGCAGTTTGTCTGACCACAGGCCTGTGATAGAGCTTCCCCA
TTGTGAGAACTCTGAAATTATCATCCGACTATATGAAATGCCTTATTTTC
CAATGGGATTTTGGTCAAGATTAATCAATCGATTACTTGAGATTTCACCT
TACATGCTTTCAGGGAGAGAACGAGCACTTCGCCCAAACAGAATGTATTG
GCGACAAGGCATTTACTTAAATTGGTCTCCTGAAGCTTATTGTCTGGTAG
GATCTGAAGTCTTAGACAATCATCCAGAGAGTTTCTTAAAAATTACAGTT
CCTTCTTGTAGAAAAGGCTGTATTCTTTTGGGCCAAGTTGTGGACCACAT
TGATTCTCTCATGGAAGAATGGTTTCCTGGGTTGCTGGAGATTGATATTT
GTGGTGAAGGAGAAACTCTGTTGAAGAAATGGGCATTATATAGTTTTAAT
GATGGTGAAGAACATCAAAAAATCTTACTTGATGACTTGATGAAGAAAGC
AGAGGAAGGAGATCTCTTAGTAAATCCAGATCAACCAAGGCTCACCATTC
CAATATCTCAGATTGCCCCTGACTTGATTTTGGCTGACCTGCCTAGAAAT
ATTATGTTGAATAATGATGAGTTGGAATTTGAACAAGCTCCAGAGTTTCT
CCTAGGTGATGGCAGTTTTGGATCAGTTTACCGAGCAGCCTATGAAGGAG
AAGAAGTGGCTGTGAAGATTTTTAATAAACATACATCACTCAGGCTGTTA
AGACAAGAGCTTGTGGTGCTTTGCCACCTCCACCACCCCAGTTTGATATC
TTTGCTGGCAGCTGGGATTCGTCCCCGGATGTTGGTGATGGAGTTAGCCT
CCAAGGGTTCCTTGGATCGCCTGCTTCAGCAGGACAAAGCCAGCCTCACT
AGAACCCTACAGCACAGGATTGCACTCCACGTAGCTGATGGTTTGAGATA
CCTCCACTCAGCCATGATTATATACCGAGACCTGAAACCCCACAATGTGC
TGCTTTTCACACTGTATCCCAATGCTGCCATCATTGCAAAGATTGCTGAC
TACAGCATTGCTCAGTACTGCTGTAGAATGGGGATAAAAACATCAGAGGG
CACACCAGGGTTTCGTGCACCTGAAGTTGCCAGAGGAAATGTCATTTATA
ACCAACAGGCTGATGTTTATTCATTTGGTTTACTACTCTATGACATTTTG
ACAACTGGAGGTAGAATAGTAGAGGGTTTGAAGTTTCCAAATGAGTTTGA
TGAATTAGAAATACAAGGAAAATTACCTGATCCAGTTAAAGAATATGGTT
GTGCCCCATGGCCTATGGTTGAGAAATTAATTAAACAGTGTTTGAAAGAA
AATCCTCAAGAAAGGCCTACTTCTGCCCAGGTCTTTGACATTTTGAATTC
AGCTGAATTAGTCTGTCTGACGAGACGCATTTTATTACCTAAAAACGTAA
TTGTTGAATGCATGGTTGCTACACATCACAACAGCAGGAATGCAAGCATT
TGGCTGGGCTGTGGGCACACCGACAGAGGACAGCTCTCATTTCTTGACTT
AAATACTGAAGGATACACTTCTGAGGAAGTTGCTGATAGTAGAATATTGT
GCTTAGCCTTGGTGCATCTTCCTGTTGAAAAGGAAAGCTGGATTGTGTCT
GGGACACAGTCTGGTACTCTCCTGGTCATCAATACCGAAGATGGGAAAAA
GAGACATACCCTAGAAAAGATGACTGATTCTGTCACTTGTTTGTATTGCA
ATTCCTTTTCCAAGCAAAGCAAACAAAAAAATTTTCTTTTGGTTGGAACC
GCTGATGGCAAGTTAGCAATTTTTGAAGATAAGACTGTTAAGCTTAAAGG
AGCTGCTCCTTTGAAGATACTAAATATAGGAAATGTCAGTACTCCATTGA
TGTGTTTGAGTGAATCCACAAATTCAACGGAAAGAAATGTAATGTGGGGA
GGATGTGGCACAAAGATTTTCTCCTTTTCTAATGATTTCACCATTCAGAA
ACTCATTGAGACAAGAACAAGCCAACTGTTTTCTTATGCAGCTTTCAGTG
ATTCCAACATCATAACAGTGGTGGTAGACACTGCTCTCTATATTGCTAAG
CAAAATAGCCCTGTTGTGGAAGTGTGGGATAAGAAAACTGAAAAACTCTG
TGGACTAATAGACTGCGTGCACTTTTTAAGGGAGGTAATGGTAAAAGAAA
ACAAGGAATCAAAACACAAAATGTCTTATTCTGGGAGAGTGAAAACCCTC
TGCCTTCAGAAGAACACTGCTCTTTGGATAGGAACTGGAGGAGGCCATAT
TTTACTCCTGGATCTTTCAACTCGTCGACTTATACGTGTAATTTACAACT
TTTGTAATTCGGTCAGAGTCATGATGACAGCACAGCTAGGAAGCCTTAAA
AATGTCATGCTGGTATTGGGCTACAACCGGAAAAATACTGAAGGTACACA
AAAGCAGAAAGAGATACAATCTTGCTTGACCGTTTGGGACATCAATCTTC
CACATGAAGTGCAAAATTTAGAAAAACACATTGAAGTGAGAAAAGAATTA
GCTGAAAAAATGAGACGAACATCTGTTGAGTAAGAGAGAAATAGGCGGCC
GC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMTQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYSIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE*
Vector Type
Baculovirus
Antibiotic
Amp
Comments
All LRRK2 plasmids MUST be grown at 30°C or less to prevent recombination Contains SNP S1647T
100042 R 1000 1001 1047 19007
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