PINK1 | LRRK2 Resource

Plasmid

pEGFP-C1-PINK1 125-581

Parent Plasmid

pEGFPC1

Expressed

GFP-PINK1 125-581

DU Number

DU3635

Genbank

BC028215

Tag

GFP

Species

Human

Sequence of Insert

View Sequence

GGATCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAAAAGCAAGCCGGG
GCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTCGGCTGGAGGAGT
ATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCTGTGTATGAA
GCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGACAAAGAGCACCGG
GTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAGGGCAGG
AGCGAGCTGCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGATGATGTGG
AACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAATGAGCCA
GGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATGGAGCAG
TCACTTACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCCCCTCACCCC
AACATCATCCGGGTTCTCCGCGCCTTCACCTCTTCCGTGCCGCTGCTGCC
AGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCCTCCACCCTG
AAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAACTATCCC
TGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCGCCTCGC
CGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGGTTCAAC
AGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCTTGTGGAGCTG
GACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGATTTTGGCTGCTGCCT
GGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCAGCTGGTACGTGG
ATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCCACGGCCCGT
CCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGCCTGGGCAGT
GGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCTTCTACGGCC
AGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAGGCTCAGCTACCT
GCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTTGGTGAGGGCACT
GCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAGTAGCCGCAAATG
TGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCCCTGAAGAATCTG
AAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATCGGCCGCCACTTT
GTTGGCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAACAAAAATGAAGA
TGCTCTTTCTGGCTAACCTGGAGTGTGAAACGCTCTGCCAGGCAGCCCTC
CTCCTCTGCTCATGGAGGGCAGCCCTGTGAGCGGCCGCGCAAGGGCGAAT
TCTGCAGATATCCATCACACTGGCGGCCGCTCGAG

Amino Acid Sequence

View Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSCQEIQA
IFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSAAVYEATMPTLPQ
NLEVTKSTGLLPGRGPGTSAPGEGQERAAGAPAFPLAIKMMWNISAGSSS
EAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRA
FTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLC
VNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWL
VIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVID
YSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPP
DVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGW
LLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAA
L*

Vector Type

Mammalian

Antibiotic

Kan

Comments

N terminal GFP for mammalian expression

100042 R 1000 1001 1047 19007