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PINK1

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Plasmid
pEBG6P-PINK1 1573-1574 ins
Parent Plasmid
pEBG6P
Expressed
GST-PINK1 1573-1574 ins
DU Number
DU3703
Genbank
BC028215
Tag
GST
Species
Human
Sequence of Insert
GGATCCGCGGTGCGACAGGCGCTGGGCCGCGGCCTGCAGCTGGGTCGAGC
GCTGCTGCTGCGCTTCACGGGCAAGCCCGGCCGGGCCTACGGCTTGGGGC
GGCCGGGCCCGGCGGCGGGCTGTGTCCGCGGGGAGCGTCCAGGCTGGGCC
GCAGGACCGGGCGCGGAGCCTCGCAGGGTCGGGCTCGGGCTCCCTAACCG
TCTCCGCTTCTTCCGCCAGTCGGTGGCCGGGCTGGCGGCGCGGTTGCAGC
GGCAGTTCGTGGTGCGGGCCTGGGGCTGCGCGGGCCCTTGCGGCCGGGCA
GTCTTTCTGGCCTTCGGGCTAGGGCTGGGCCTCATCGAGGAAAAACAGGC
GGAGAGCCGGCGGGCGGTCTCGGCCTGTCAGGAGATCCAGGCAATTTTTA
CCCAGAAAAGCAAGCCGGGGCCTGACCCGTTGGACACGAGACGCTTGCAG
GGCTTTCGGCTGGAGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTG
CAGTGCTGCTGTGTATGAAGCCACCATGCCTACATTGCCCCAGAACCTGG
AGGTGACAAAGAGCACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGT
GCACCAGGAGAAGGGCAGGAGCGAGCTGCGGGGGCCCCTGCCTTCCCCTT
GGCCATCAAGATGATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCA
TCTTGAACACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTG
GCTGGGGAGTATGGAGCAGTCACTTACAGAAAATCCAAGAGAGGTCCCAA
GCAACTAGCCCCTCACCCCAACATCATCCGGGTTCTCCGCGCCTTCACCT
CTTCCGTGCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTG
CCCTCACGCCTCCACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCT
CGTTATGAAGAACTATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACA
CACCCAGCCCCCGCCTCGCCGCCATGATGCTGCTGCAGCTGCTGGAAGGC
GTGGACCATCTGGTTCAACAGGGCATCGCGCACAGAGACCTGAAATCCGA
CAACATCCTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGCTGGTGATCG
CAGATTTTGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCC
TTCAGCAGCTGGTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCC
AGAGGTGTCCACGGCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCA
AGGCTGATGCCTGGGCAGTGGGAGCCATCGCCTATGAAATCTTCGGGCTT
GTCAATCCCTTCTACGGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGCTA
CCAAGAGGCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTCCAGACGTGA
GACAGTTGGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCT
GCCCGAGTAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGGTGAACATAT
TCTAGCCCTGAAGAATCTGAAGTTATTAGGACAAGATGGTTGGCTGGCTC
CTCCAACAATCGGCCGCCACTTTGTTGGCCAACAGGCTCACAGAGAAGTG
TTGTGTGGAAACAAAAATGAAGATGCTCTTTCTGGCTAACCTGGAGTGTG
AAACGCTCTGCCAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAGCCCTG
TGAGCGGCCGCGCAAGGGCGAATTCCAGCACACTGGCGGCCGTTACTAGT
GGATCC
Amino Acid Sequence
MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSAVRQALGRGLQLG
RALLLRFTGKPGRAYGLGRPGPAAGCVRGERPGWAAGPGAEPRRVGLGLP
NRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAVFLAFGLGLGLIEEK
QAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGK
GCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAAGAPAF
PLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRG
PKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTL
FLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLK
SDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLM
APEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESR
SYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGE
HILALKNLKLLGQDGWLAPPTIGRHFVGQQAHREVLCGNKNEDALSG*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N terminal GST for mammalian expression. Insertion causes frame change after aa 524 leading to truncation.
100042 R 1000 1001 1047 19007
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