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Plasmid
pEBG6P-PINK1 125-539 D384A
Parent Plasmid
pEBG6P
Expressed
GST-PINK1 125-539 D384A
DU Number
DU3598
Genbank
BC028215
Tag
GST
Species
Human
Sequence of Insert
View Sequence
GGATCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAAAAGCAAGCCGGG
GCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTCGGCTGGAGGAGT
ATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCTGTGTATGAA
GCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGACAAAGAGCACCGG
GTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAGGGCAGG
AGCGAGCTGCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGATGATGTGG
AACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAATGAGCCA
GGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATGGAGCAG
TCACTTACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCCCCTCACCCC
AACATCATCCGGGTTCTCCGCGCCTTCACCTCTTCCGTGCCGCTGCTGCC
AGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCCTCCACCCTG
AAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAACTATCCC
TGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCGCCTCGC
CGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGGTTCAAC
AGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCTTGTGGAGCTG
GACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGCTTTTGGCTGCTGCCT
GGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCAGCTGGTACGTGG
ATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCCACGGCCCGT
CCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGCCTGGGCAGT
GGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCTTCTACGGCC
AGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAGGCTCAGCTACCT
GCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTTGGTGAGGGCACT
GCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAGTAGCCGCAAATG
TGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCCCTGAAGAATCTG
AAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATCGGCCGCCACTTT
GTAAGCGGCCGC
GCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTCGGCTGGAGGAGT
ATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCTGTGTATGAA
GCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGACAAAGAGCACCGG
GTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAGGGCAGG
AGCGAGCTGCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGATGATGTGG
AACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAATGAGCCA
GGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATGGAGCAG
TCACTTACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCCCCTCACCCC
AACATCATCCGGGTTCTCCGCGCCTTCACCTCTTCCGTGCCGCTGCTGCC
AGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCCTCCACCCTG
AAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAACTATCCC
TGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCGCCTCGC
CGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGGTTCAAC
AGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCTTGTGGAGCTG
GACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGCTTTTGGCTGCTGCCT
GGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCAGCTGGTACGTGG
ATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCCACGGCCCGT
CCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGCCTGGGCAGT
GGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCTTCTACGGCC
AGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAGGCTCAGCTACCT
GCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTTGGTGAGGGCACT
GCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAGTAGCCGCAAATG
TGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCCCTGAAGAATCTG
AAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATCGGCCGCCACTTT
GTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSCQEIQAIFTQKSK
PGPDPLDTRRLQGFRLEEYLIGQSIGKGCSAAVYEATMPTLPQNLEVTKS
TGLLPGRGPGTSAPGEGQERAAGAPAFPLAIKMMWNISAGSSSEAILNTM
SQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAFTSSVPL
LPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPR
LAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIAAFGC
CLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAW
AVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVR
ALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAA
TL*
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSCQEIQAIFTQKSK
PGPDPLDTRRLQGFRLEEYLIGQSIGKGCSAAVYEATMPTLPQNLEVTKS
TGLLPGRGPGTSAPGEGQERAAGAPAFPLAIKMMWNISAGSSSEAILNTM
SQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAFTSSVPL
LPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPR
LAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIAAFGC
CLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAW
AVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVR
ALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAA
TL*
Vector Type
Mammalian
Antibiotic
Amp
Comments
N terminal GST for mammalian expression.Kinase dead mutation.
100042 R 1000 1001 1047 19007
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