PINK1 | LRRK2 Resource

Plasmid

pEBG6P-PINK1 2-539

Parent Plasmid

pEBG6P

Expressed

GST-PINK1 2-539

DU Number

DU3822

Genbank

BC028215

Tag

GST

Species

Human

Sequence of Insert

View Sequence

GGATCCGCGGTGCGACAGGCGCTGGGCCGCGGCCTGCAGCTGGGTCGAGC
GCTGCTGCTGCGCTTCACGGGCAAGCCCGGCCGGGCCTACGGCTTGGGGC
GGCCGGGCCCGGCGGCGGGCTGTGTCCGCGGGGAGCGTCCAGGCTGGGCC
GCAGGACCGGGCGCGGAGCCTCGCAGGGTCGGGCTCGGGCTCCCTAACCG
TCTCCGCTTCTTCCGCCAGTCGGTGGCCGGGCTGGCGGCGCGGTTGCAGC
GGCAGTTCGTGGTGCGGGCCTGGGGCTGCGCGGGCCCTTGCGGCCGGGCA
GTCTTTCTGGCCTTCGGGCTAGGGCTGGGCCTCATCGAGGAAAAACAGGC
GGAGAGCCGGCGGGCGGTCTCGGCCTGTCAGGAGATCCAGGCAATTTTTA
CCCAGAAAAGCAAGCCGGGGCCTGACCCGTTGGACACGAGACGCTTGCAG
GGCTTTCGGCTGGAGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTG
CAGTGCTGCTGTGTATGAAGCCACCATGCCTACATTGCCCCAGAACCTGG
AGGTGACAAAGAGCACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGT
GCACCAGGAGAAGGGCAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTT
GGCCATCAAGATGATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCA
TCTTGAACACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTG
GCTGGGGAGTATGGAGCAGTCACTTACAGAAAATCCAAGAGAGGTCCCAA
GCAACTAGCCCCTCACCCCAACATCATCCGGGTTCTCCGCGCCTTCACCT
CTTCCGTGCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTG
CCCTCACGCCTCCACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCT
CGTTATGAAGAACTATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACA
CACCCAGCCCCCGCCTCGCCGCCATGATGCTGCTGCAGCTGCTGGAAGGC
GTGGACCATCTGGTTCAACAGGGCATCGCGCACAGAGACCTGAAATCCGA
CAACATCCTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGCTGGTGATCG
CAGATTTTGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCC
TTCAGCAGCTGGTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCC
AGAGGTGTCCACGGCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCA
AGGCTGATGCCTGGGCAGTGGGAGCCATCGCCTATGAAATCTTCGGGCTT
GTCAATCCCTTCTACGGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGCTA
CCAAGAGGCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTCCAGACGTGA
GACAGTTGGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCT
GCCCGAGTAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGGTGAACATAT
TCTAGCCCTGAAGAATCTGAAGTTAGACAAGATGGTTGGCTGGCTCCTCC
AACAATCGGCCGCCACTTTGTAAGCGGCCGC

Amino Acid Sequence

View Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSAVRQALGRGLQLG
RALLLRFTGKPGRAYGLGRPGPAAGCVRGERPGWAAGPGAEPRRVGLGLP
NRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAVFLAFGLGLGLIEEK
QAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGK
GCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAF
PLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRG
PKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTL
FLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLK
SDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLM
APEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESR
SYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGE
HILALKNLKLDKMVGWLLQQSAATL*

Vector Type

Mammalian

Antibiotic

Amp

Comments

N terminal GST for mammalian expression.
Calc Mol Weight : 85272 Daltons

100042 R 1000 1001 1047 19007