RAB11-FIP1, RFIP1 | LRRK2 Resource

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Synonyms

rab11 family-interacting protein 1

Plasmid

pGEX6P-1-RAB11FIP1 isoform 3

Parent Plasmid

pGEX6P-1

Expressed

GST-RAB11FIP1 isoform 3

DU Number

DU28725

Genbank

NM_001002814.2 → NP_001002814.2

Tag

GST

Species

Human

Sequence of Insert

View Sequence

ggatccATGTCCCTAATGGTCTCGGCTGGCCGGGGCCTGGGGGCCGTGTG
GTCCCCAACCCACGTGCAGGTGACGGTGCTGCAGGCGCGGGGCCTGCGGG
CCAAGGGCCCCGGGGGCACGAGCGACGCGTACGCGGTGATCCAGGTGGGC
AAGGAGAAGTACGCCACCTCCGTGTCGGAGCGCAGCCTGGGCGCGCCCGT
GTGGCGCGAGGAGGCCACCTTCGAGCTGCCATCGCTGCTGTCCTCCGGAC
CCGCGGCCGCCGCCACCCTGCAGCTCACCGTGCTGCACCGCGCGCTGCTC
GGCCTCGACAAGTTCCTGGGCCGCGCCGAGGTGGACCTGCGGGATCTGCA
CCGCGACCAGGGCCGCAGGAAGACGCAGTGGTATAAGTTGAAATCCAAAC
CAGGAAAGAAGGACAAGGAGCGAGGAGAAATTGAGGTTGACATCCAGTTT
ATGAGAAACAACATGACTGCCAGCATGTTTGACCTTTCTATGAAAGACAA
GTCTCGGAATCCATTTGGAAAGCTGAAGGACAAGATCAAGGGGAAGAATA
AGGACAGTGGGTCAGACACCGCCTCCGCCATCATCCCTAGCACGACACCT
TCGGTCGACAGTGATGATGAGTCTGTGGTTAAAGACAAGAAAAAGAAATC
AAAGATCAAGACCTTACTTTCCAAGTCAAATTTGCAGAAGACGCCTCTTT
CCCAGTCCATGTCTGTCCTGCCGACTTCAAAGCCAGAAAAAGTGCTGCTT
CGTCCCGGAGACTTTCAGTCCCAGTGGGATGAAGATGACAATGAGGATGA
GTCCTCCTCGGCCTCGGATGTCATGTCTCACAAGAGAACAGCGAGTACGG
ATCTTAAGCAACTGAACCAGGTCAACTTTACCCTTCCCAAGAAGGAAGGA
CTTTCCTTTCTTGGTGGCCTTCGGTCTAAGAATGATGTCCTTTCCCGCTC
TAATGTCTGCATCAATGGGAACCATGTTTACCTGGAGCAGCCAGAAGCCA
AGGGTGAGATCAAGGATAGCAGCCCGTCCTCCTCCCCATCCCCCAAGGGC
TTCAGAAAGAAGCATTTGTTCTCTTCTACAGAGAACCTGGCGGCTGGGTC
TTGGAAGGAGCCTGCTGAAGGAGGTGGGCTGTCTTCTGACAGGCAGCTCT
CCGAATCTTCCACCAAGGACTCCTTGAAGTCTATGACCCTGCCGTCCTAC
CGACCTGCCCCACTGGTCAGTGGGGACCTCAGGGAAAACATGGCCCCCGC
AAACTCAGAGGCCACAAAAGAAGCTAAGGAGAGCAAGAAGCCAGAGAGCA
GGAGGTCCTCTTTGCTGTCTCTGATGACGGGGAAGAAGGATGTGGCTAAG
GGCAGTGAAGGTGAAAACCCTCTCACGGTCCCAGGGAGGGAGAAGGAAGG
CATGCTGATGGGGGTTAAGCCGGGGGAGGACGCATCGGGGCCTGCTGAAG
ACCTTGTGAGAAGATCTGAGAAAGATACTGCAGCTGTTGTCTCCAGACAG
GGCAGCTCCCTGAACCTCTTTGAAGATGTGCAGATCACAGAACCAGAAGC
TGAGCCAGAGTCCAAGTCTGAACCGAGACCTCCAATTTCCTCTCCGAGGG
CTCCCCAGACCAGAGCTGTCAAGCCCCGACTGGAAGTGTCTCCAGAGGCG
CAACCCACAGCCAGGCTTCCTTCCCCTACTGACTCCCCTTCCTCTCTTCC
TCCTCTTCCTTCTAGCTCTGGCCAGGCATCTGTCCCCTCTGAATTGGGAC
ATGGTGCAGACACACAGTCCTCTGAGAGTCCTTCTGTCTTCTCCTCTCTC
TCATCTCCCATAGCAGCTCCCATTTCCACATCCACTCCAATTGAAAGCTG
GCCTCTCGTAGACAGGGGCAAGGCCAAGTCTGAAGGACCACCCTTGCTCC
CTAAGGCAGAGTTGCAAACTGAGAGTTTAACACCGGTTCCAAATTCTGGT
TCTTCTGCCCTGGGATCACTTTTCAAACAGCCATCCTTTCCAGCAAATAA
AGGGACAGAAGATTCTCTGATGGGCAGGACCCGTGAGACAGGCACAGAGA
AGAACACCAGCAGCCTTGAGTTGGAGGAGTCTCTCCCAGAGCAGCCTGAA
ACAGGGCGACAAGAGGAAGAACTTCCGAGATTCCCCTGCAAAAAACAAGA
CTACAGCCCATCATCTGGAGAAGCCCAGGAAGTACCGTTTGCCCTTTCAC
TCAGCAGTGATGGAGCTGTGAGCCCTGTTGGGGAGCTTGCAGCAGGAGGA
GACAGAGACTTGGAGAGTCAGGCTGGGTCTCTTGTGGAGAGCAAAGCCAG
GGATGCAGCTGAAGAAGTGGCGCCCCCTCTTCCCATGGGAGCATCAGTCC
CTTCCATTGATTCCATGATGCGGAAGCTGGAAGAGATGGGTCTGAACCTC
CGCAAGGACCAGAAGAAAACCAAGAAGCGTGTGTCATTTTCTGAGCAGCT
CTTCACGGAAGAGGCAGTGGCGGGGGCTGCCTTGCTGGTGGAAGGACACA
GCAGTTGTCCCCAGGAGCTGAACCCTGCATGGTCTGTTGCTGGAAACGCG
TCTGACGGAGAGCCTCCTGAGTCTCCCCACGCAGAGGACTCAGAAAGGGA
ATCGGTGACCACACCTGGGCCAGCGACGTGTGGTGCGCCAGCCTCCCCAG
CGGATCACCTCCTCCTCCCCTCCCAGGAGGAGAGTTTCTCCGAAGTCCCC
ATGAGTGAAGCAAGCTCAGCGAAAGACACTCCACTCTTTAGGATGGAGGG
AGAGGATGCCCTTGTGACTCAGTATCAGAGCAAAGCCAGTGACCACGAAG
GTTTATTGTCTGACCCCTTGAGTGACCTTCAGTTGGTCTCAGATTTTAAA
TCTCCAATCATGGCCGATCTGAACTTAAGCCTTCCTTCCATTCCTGAAGT
CGCATCGGATGATGAAAGAATAGATCAGGTTGAAGATGACGGAGATCAGG
TTGAAGATGATGGAGAGACAGCAAAGTCGTCAACTCTGGACATAGGAGCT
TTGTCCTTGGGCTTGGTAGTCCCCTGTCCTGAGAGGGGAAAGGGGCCCAG
TGGCGAGGCAGATAGGTTGGTACTGGGGGAGGGCCTGTGTGATTTCAGGC
TGCAAGCACCCCAGGCATCTGTGACAGCTCCTTCAGAGCAGACCACAGAG
TTCGGAATTCACAAACCACATCTTGGCAAGAGCTCAAGCTTGGATAAACA
GCTGCCAGGCCCCAGTGGTGGTGAGGAAGAAAAACCGATGGGAAATGGGA
GTCCAAGCCCGCCTCCTGGCACATCCCTGGACAATCCTGTACCCAGCCCC
TCCCCTTCTGAGATCTTTCCTGTCACACACTCTTTCCCCAGCTCTGCACA
TTCTGACACTCACCACACCAGCACAGCAGAATCTCAAAAAAAAGCCACAG
CAGAGGGCTCCGCTGGTAGAGTTGAAAATTTTGGCAAGAGGAAGCCACTC
CTCCAGGCCTGGGTCTCACCCTCGGAGACACATCCAGTCTCAGCTCAGCC
AGGCGCTGGAACTGGGTCAGCCAAGCACAGACTTCATCCTGTGAAGCCAA
TGAATGCAATGGCCACCAAGGTTGCTAACTGCAGCTTGGGAACTGCCACC
ATCATCAGTGAGAACTTGAACAATGAGGTCATGATGAAGAAATACAGCCC
CTCGGACCCTGCATTTGCATATGCGCAGCTGACCCACGATGAGCTGATTC
AGCTGGTCCTCAAACAGAAGGAAACGATAAGCAAGAAGGAGTTCCAGGTC
CGCGAGCTGGAAGACTACATTGACAACCTGCTTGTCAGGGTCATGGAAGA
AACCCCCAATATCCTCCGCATCCCGACTCAGGTTGGCAAAAAAGCAGGAA
AGATGTAActcgag

Amino Acid Sequence

View Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSLMVSAGRGLGAVWSPTH
VQVTVLQARGLRAKGPGGTSDAYAVIQVGKEKYATSVSERSLGAPVWREE
ATFELPSLLSSGPAAAATLQLTVLHRALLGLDKFLGRAEVDLRDLHRDQG
RRKTQWYKLKSKPGKKDKERGEIEVDIQFMRNNMTASMFDLSMKDKSRNP
FGKLKDKIKGKNKDSGSDTASAIIPSTTPSVDSDDESVVKDKKKKSKIKT
LLSKSNLQKTPLSQSMSVLPTSKPEKVLLRPGDFQSQWDEDDNEDESSSA
SDVMSHKRTASTDLKQLNQVNFTLPKKEGLSFLGGLRSKNDVLSRSNVCI
NGNHVYLEQPEAKGEIKDSSPSSSPSPKGFRKKHLFSSTENLAAGSWKEP
AEGGGLSSDRQLSESSTKDSLKSMTLPSYRPAPLVSGDLRENMAPANSEA
TKEAKESKKPESRRSSLLSLMTGKKDVAKGSEGENPLTVPGREKEGMLMG
VKPGEDASGPAEDLVRRSEKDTAAVVSRQGSSLNLFEDVQITEPEAEPES
KSEPRPPISSPRAPQTRAVKPRLEVSPEAQPTARLPSPTDSPSSLPPLPS
SSGQASVPSELGHGADTQSSESPSVFSSLSSPIAAPISTSTPIESWPLVD
RGKAKSEGPPLLPKAELQTESLTPVPNSGSSALGSLFKQPSFPANKGTED
SLMGRTRETGTEKNTSSLELEESLPEQPETGRQEEELPRFPCKKQDYSPS
SGEAQEVPFALSLSSDGAVSPVGELAAGGDRDLESQAGSLVESKARDAAE
EVAPPLPMGASVPSIDSMMRKLEEMGLNLRKDQKKTKKRVSFSEQLFTEE
AVAGAALLVEGHSSCPQELNPAWSVAGNASDGEPPESPHAEDSERESVTT
PGPATCGAPASPADHLLLPSQEESFSEVPMSEASSAKDTPLFRMEGEDAL
VTQYQSKASDHEGLLSDPLSDLQLVSDFKSPIMADLNLSLPSIPEVASDD
ERIDQVEDDGDQVEDDGETAKSSTLDIGALSLGLVVPCPERGKGPSGEAD
RLVLGEGLCDFRLQAPQASVTAPSEQTTEFGIHKPHLGKSSSLDKQLPGP
SGGEEEKPMGNGSPSPPPGTSLDNPVPSPSPSEIFPVTHSFPSSAHSDTH
HTSTAESQKKATAEGSAGRVENFGKRKPLLQAWVSPSETHPVSAQPGAGT
GSAKHRLHPVKPMNAMATKVANCSLGTATIISENLNNEVMMKKYSPSDPA
FAYAQLTHDELIQLVLKQKETISKKEFQVRELEDYIDNLLVRVMEETPNI
LRIPTQVGKKAGKM*

Vector Type

Bacterial

Antibiotic

Amp

Comments

Parental 28701. 2.2kb Sph1-Nde1 (native sites) RT-PCR product sub cloned from 28682 into 28701 pGEX intermediate to create isoform 3 (flanked Bam-Xho). Contains known Q622K SNP

100042 R 1000 1001 1047 19007

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