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Full Name
RAB5A phospho Ser 84
Long Name Textual
RAB5A, member RAS oncogene family
Immuno Sequence
AGQERYHS*LAPMYYR [residues 77 - 91 of human]
Mono/Poly clonal
Polyclonal
Sheep No
S942D
Aliquot
0.1mg
100041 R 1000 1001 1047 19007
Gel Image
Gel Description
HEK293 LRRK2 [R1441G] FlpIn cells were transfected with pCMV5 HA-Rab5A and after 24 h of incubation expression of LRRK2 [R1441G] was induced using tetracycline (TET). After 24 h the cells were treated for 90 minutes with 100 nM MLi2 (Lrrk2 inhibitor) before lysis. 20 ug of the lysates was for SDS-PAGE and blotting. The antibodies were used in 5% milk in TBST at 1 ug/ml and 10 ug/ml of corresponding non-phospho peptide. The membranes were incubated with the antibodies overnight in 4°C. The secondary sheep antibody conjugated with HRP was used in 5% milk in TBST for 1 hour at RT.