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Plasmid
pET15b 6HIS SUMO JIP3
Parent Plasmid
pET15b 6His SUMO
Expressed
6HIS SUMO JIP3
DU Number
DU27813
Genbank
NP_055948.2. NM_015133.4. [Q9UPT6-1]
Tag
6His
SUMO
Species
Human
Sequence of Insert
View Sequence
ATGATGGAGATCCAGATGGACGAGGGCGGCGGCGTGGTGGTGTACCAGGACGACTACTGCTCCGGCTCGGTGATGTCGGAGCGGGTGTCGGGCCTGGCGGGCTCCATCTACCGCGAGTTCGAGCGCCTCATCCACTGCTACGACGAGGAGGTGGTCAAGGAGCTCATGCCGCTGGTGGTGAACGTGCTGGAGAACCTAGACTCGGTGCTCAGCGAGAACCAGGAGCACGAGGTGGAGCTGGAGCTGCTGCGCGAGGACAACGAGCAGCTGCTCACCCAGTACGAGCGTGAGAAGGCGCTGCGCAGGCAGGCGGAGGAGAAATTCATTGAGTTTGAAGATGCTCTGGAACAAGAGAAGAAAGAGCTGCAAATCCAGGTGGAGCACTACGAGTTCCAGACGCGCCAGCTGGAGCTGAAGGCCAAGAACTATGCCGATCAGATTTCCCGGTTGGAGGAGCGGGAGTCGGAGATGAAGAAGGAGTACAATGCCCTGCACCAGCGGCACACAGAGATGATACAGACCTACGTGGAGCACATTGAGAGGTCCAAGATGCAGCAGGTCGGAGGAAACAGCCAGACCGAGAGCAGCCTGCCGGGGCGGAGGAAGGAGCGCCCCACCTCCCTGAACGTGTTCCCCCTGGCTGACGGCACGGTACGTGCACAGATCGGGGGCAAGCTCGTGCCTGCGGGGGACCACTGGCACCTGAGTGACCTCGGCCAGCTGCAGTCCAGCTCCAGCTACCAGTGTCCACAGGATGAAATGTCCGAGTCAGGCCAGTCCTCGGCGGCCGCCACACCCAGCACCACAGGCACCAAGTCCAACACGCCCACATCCTCCGTGCCCTCGGCCGCCGTCACACCCCTCAACGAGAGCCTGCAGCCCCTGGGGGACTATGGCGTGGGCTCCAAGAACAGCAAGCGTGCCCGGGAGAAGCGCGACAGCCGCAACATGGAAGTACAGGTCACCCAGGAGATGCGCAACGTCAGTATAGGCATGGGCAGCAGTGACGAGTGGTCTGATGTTCAAGACATTATTGACTCCACGCCAGAGCTGGACATGTGTCCAGAGACCCGCCTGGACCGCACAGGAAGCAGCCCAACCCAGGGCATCGTGAACAAAGCTTTCGGCATCAACACCGACTCCCTGTACCATGAGCTGTCGACGGCAGGGTCTGAGGTCATCGGGGATGTGGACGAAGGGGCCGACCTCCTAGGGGAGTTCTCAGTGCGCGATGATTTCTTTGGAATGGGCAAAGAAGTGGGGAATCTGCTACTGGAAAACTCACAGCTTCTGGAAACCAAAAACGCCTTGAATGTGGTGAAGAATGACCTGATTGCCAAGGTCGACCAGCTGTCCGGGGAGCAGGAGGTGCTGAGGGGCGAGTTGGAGGCTGCTAAGCAGGCCAAAGTCAAGCTGGAAAACCGTATCAAGGAGCTGGAAGAGGAACTGAAAAGAGTGAAGTCCGAGGCCATCATCGCCCGCCGTGAACCCAAAGAAGAGGCGGAGGATGTAAGCAGCTATCTCTGTACAGAATCGGACAAAATCCCCATGGCCCAGCGCCGCCGCTTCACGCGGGTGGAGATGGCCCGTGTGCTCATGGAGCGGAACCAGTACAAGGAGCGGCTGATGGAGCTGCAGGAGGCTGTGCGGTGGACTGAGATGATCAGAGCGTCCCGAGAGCACCCATCCGTCCAGGAGAAGAAGAAGTCGACCATCTGGCAGTTCTTCAGCCGCCTCTTCAGCTCTTCCTCCAGCCCCCCTCCGGCCAAGCGCCCCTATCCCTCGGTGAACATCCACTACAAGTCACCCACCACTGCCGGCTTCAGCCAGCGCCGCAACCATGCCATGTGCCCGATCTCGGCAGGCAGCCGGCCCCTGGAATTCTTCCCTGACGACGACTGCACGTCCTCCGCCCGTCGAGAGCAGAAGCGCGAGCAGTACCGCCAGGTGCGTGAGCACGTGCGTAACGACGACGGCCGTCTGCAGGCCTGCGGCTGGAGCCTGCCCGCCAAGTACAAGCAGCTGAGTCCCAACGGGGGCCAGGAGGACACGCGGATGAAGAACGTGCCGGTGCCGGTGTACTGCCGCCCTCTGGTGGAGAAGGACCCCACCATGAAGCTGTGGTGTGCCGCGGGCGTCAACCTGAGCGGGTGGAGGCCCAATGAGGACGACGCTGGGAATGGAGTCAAGCCAGCGCCAGGCCGCGATCCCCTGACCTGCGACCGCGAAGGAGACGGCGAGCCCAAGAGCGCCCACACGTCTCCCGAGAAGAAGAAGGCCAAGGAGCTCCCTGAAATGGACGCCACCTCCAGCCGGGTGTGGATCCTGACCAGCACCCTGACCACCAGCAAGGTGGTGATCATCGACGCCAACCAGCCGGGCACGGTGGTGGACCAGTTCACCGTCTGCAACGCGCACGTGCTGTGCATCTCCAGCATCCCCGCGGCCAGCGACAGCGACTACCCTCCCGGGGAGATGTTCCTGGACAGCGACGTGAACCCAGAGGACCCGGGCGCAGATGGCGTGCTGGCCGGTATCACCCTGGTGGGCTGTGCCACCCGCTGCAACGTGCCGCGGAGCAACTGCTCCTCCCGAGGGGACACCCCAGTGCTAGACAAGGGGCAGGGGGAGGTGGCCACCATCGCCAACGGGAAGGTCAACCCGTCCCAGTCCACAGAGGAGGCCACAGAGGCCACGGAGGTGCCAGACCCTGGGCCCAGCGAGCCAGAGACAGCCACATTGCGGCCCGGGCCTCTCACAGAGCACGTCTTCACTGACCCAGCCCCGACCCCGTCCTCTGGCCCCCAGCCTGGCAGCGAGAACGGGCCAGAGCCTGACAGCAGCAGCACACGGCCAGAGCCAGAGCCCAGCGGGGACCCCACGGGAGCAGGCAGCAGTGCTGCACCCACCATGTGGCTGGGAGCCCAGAACGGCTGGCTCTATGTGCACTCGGCTGTGGCCAACTGGAAGAAGTGCCTGCACTCCATCAAGCTGAAGGATTCTGTGCTGAGCCTGGTGCATGTCAAAGGCCGTGTGCTGGTGGCTCTGGCGGACGGGACCCTGGCCATCTTCCACCGTGGTGAAGATGGCCAGTGGGATCTGAGCAACTATCACCTAATGGACCTGGGCCACCCGCACCACTCCATCCGCTGCATGGCTGTTGTGTACGACCGCGTGTGGTGTGGCTACAAGAACAAGGTGCACGTCATCCAGCCCAAGACCATGCAGATAGAGAAGTCATTTGACGCCCACCCGCGGCGGGAGAGCCAGGTGCGGCAGCTGGCGTGGATCGGCGATGGCGTATGGGTGTCCATCCGCCTGGACTCCACCCTGAGGCTCTACCATGCACACACGCACCAGCATCTACAGGACGTGGACATTGAGCCCTACGTCAGCAAGATGCTAGGCACTGGCAAGCTGGGTTTCTCCTTCGTACGCATCACGGCCCTGCTTGTCGCGGGCAGCCGGCTCTGGGTGGGCACCGGCAACGGAGTGGTCATCTCCATCCCCCTGACAGAGACTGTGGTCCTGCACCGAGGCCAGCTCCTGGGGCTCCGAGCCAATAAGACATCCCCCACCTCTGGGGAGGGCGCCCGTCCCGGGGGCATCATCCACGTGTATGGCGATGACAGCAGTGACAGGGCGGCCAGCAGCTTCATCCCCTACTGCTCCATGGCCCAGGCCCAGCTATGCTTCCATGGGCACCGCGATGCCGTGAAGTTCTTTGTCTCGGTGCCAGGGAACGTGCTGGCCACCCTGAATGGGAGTGTGCTGGACAGCCCAGCCGAGGGCCCTGGGCCAGCTGCCCCTGCCTCGGAGGTCGAGGGCCAGAAGCTGCGGAACGTGCTGGTGCTGAGCGGCGGGGAGGGCTACATCGACTTCCGCATTGGAGACGGAGAGGACGACGAGACGGAGGAGGGCGCAGGGGACATGAGCCAGGTGAAGCCCGTGCTGTCCAAGGCAGAGCGCAGTCACATCATCGTGTGGCAGGTGTCCTACACCCCCGAGTGA
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGMMEIQMDEGGGVVVYQDDYCSGSVMSERVSGLAGSIYREFERLIHCYDEEVVKELMPLVVNVLENLDSVLSENQEHEVELELLREDNEQLLTQYEREKALRRQAEEKFIEFEDALEQEKKELQIQVEHYEFQTRQLELKAKNYADQISRLEERESEMKKEYNALHQRHTEMIQTYVEHIERSKMQQVGGNSQTESSLPGRRKERPTSLNVFPLADGTVRAQIGGKLVPAGDHWHLSDLGQLQSSSSYQCPQDEMSESGQSSAAATPSTTGTKSNTPTSSVPSAAVTPLNESLQPLGDYGVGSKNSKRAREKRDSRNMEVQVTQEMRNVSIGMGSSDEWSDVQDIIDSTPELDMCPETRLDRTGSSPTQGIVNKAFGINTDSLYHELSTAGSEVIGDVDEGADLLGEFSVRDDFFGMGKEVGNLLLENSQLLETKNALNVVKNDLIAKVDQLSGEQEVLRGELEAAKQAKVKLENRIKELEEELKRVKSEAIIARREPKEEAEDVSSYLCTESDKIPMAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASREHPSVQEKKKSTIWQFFSRLFSSSSSPPPAKRPYPSVNIHYKSPTTAGFSQRRNHAMCPISAGSRPLEFFPDDDCTSSARREQKREQYRQVREHVRNDDGRLQACGWSLPAKYKQLSPNGGQEDTRMKNVPVPVYCRPLVEKDPTMKLWCAAGVNLSGWRPNEDDAGNGVKPAPGRDPLTCDREGDGEPKSAHTSPEKKKAKELPEMDATSSRVWILTSTLTTSKVVIIDANQPGTVVDQFTVCNAHVLCISSIPAASDSDYPPGEMFLDSDVNPEDPGADGVLAGITLVGCATRCNVPRSNCSSRGDTPVLDKGQGEVATIANGKVNPSQSTEEATEATEVPDPGPSEPETATLRPGPLTEHVFTDPAPTPSSGPQPGSENGPEPDSSSTRPEPEPSGDPTGAGSSAAPTMWLGAQNGWLYVHSAVANWKKCLHSIKLKDSVLSLVHVKGRVLVALADGTLAIFHRGEDGQWDLSNYHLMDLGHPHHSIRCMAVVYDRVWCGYKNKVHVIQPKTMQIEKSFDAHPRRESQVRQLAWIGDGVWVSIRLDSTLRLYHAHTHQHLQDVDIEPYVSKMLGTGKLGFSFVRITALLVAGSRLWVGTGNGVVISIPLTETVVLHRGQLLGLRANKTSPTSGEGARPGGIIHVYGDDSSDRAASSFIPYCSMAQAQLCFHGHRDAVKFFVSVPGNVLATLNGSVLDSPAEGPGPAAPASEVEGQKLRNVLVLSGGEGYIDFRIGDGEDDETEEGAGDMSQVKPVLSKAERSHIIVWQVSYTPE*
Vector Type
Mammalian
Antibiotic
Amp
Comments
linker after SENP cleavage deleted to make flush
100042 R 1000 1001 1047 19007
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