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Plasmid
pET15b SUMOb RAB12 Q101L
Parent Plasmid
pET15b 6His SUMO
Expressed
6HIS-SUMOb-RAB12 Q101L
DU Number
DU52258
Genbank
NM_001025300.2
Tag
6His
SUMO
Species
Human
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMDPGAALQRRAGGGGGLGAGSPALSGGQGRRRKQPPRPADFKLQVIIIGSRGVGKTSLMERFTDDTFCEACKSTVGVDFKIKTVELRGKKIRLQIW
DTAGLERFNSITSAYYRSAKGIILVYDITKKETFDDLPKWMKMIDKYASEDAELLLVGNKLDCETDREITRQQGEKFAQQITGMRFCEASAKDNFNVDEI
FLKLVDDILKKMPLDILRNELSNSILSLQPEPEIPPELPPPRPHVRCC*
QTGGMDPGAALQRRAGGGGGLGAGSPALSGGQGRRRKQPPRPADFKLQVIIIGSRGVGKTSLMERFTDDTFCEACKSTVGVDFKIKTVELRGKKIRLQIW
DTAGLERFNSITSAYYRSAKGIILVYDITKKETFDDLPKWMKMIDKYASEDAELLLVGNKLDCETDREITRQQGEKFAQQITGMRFCEASAKDNFNVDEI
FLKLVDDILKKMPLDILRNELSNSILSLQPEPEIPPELPPPRPHVRCC*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Q101L and silent T>C base change at position 6. SUMO blunt mutated via serine deletion in BamHI site.
100042 R 1000 1001 1047 19007
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