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Full Name
PINK (186 - 202)
Long Name Textual
PTEN induced putative kinase 1
Immuno Sequence
KSTGLLPGRGPGTSAPG [residues 186 - 202 of human]
Mono/Poly clonal
Polyclonal
Sheep No
S688B
Aliquot
0.1mg
100041 R 1000 1001 1047 19007
Gel Image
Gel Description
HEK293 cells were either untransfected (lane one) or transfected with full length (1-581) PINK1-myc cDNA (lane two). 25μg of protein was subjected to SDS-PAGE analysis before being transferred to nitrocellulose membrane and immunoblotted with anti-PINK1 (186-202) at 1 μg/ml overnight at 4°C. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 2500 dilution) and enhanced chemiluminescence.
PINK1 was immunoprecipitated from 1mg HEK 293 cellular extracts over-expressing humanwildtypePINK1. Immunoprecipitateswereperformedusing5μgofpre-immune serum covalently coupled to protein G sepharose (lane 1); 5μg anti-PINK1 S688B antibody covalently coupled to protein G sepharose (lane 2); 5μg anti-PINK1 S460C (2nd bleed) antibody covalently coupled to protein G sepharose (lane 3); 5μg anti-PINK1 S460C (3rd bleed) antibody covalently coupled to protein G sepharose (lane 4) or 5μg anti-PINK1 S514C antibody covalently coupled to protein G sepharose (lane 5). Immunoprecipitates were subjected to SDS-PAGE analysis before being transferred to nitrocellulose membrane and immunoblotted with anti-PINK1 S460C antibody at 1 μg/ml overnight at 4°C. Binding of the primary antibody was detected using rabbit peroxidase conjugated anti- sheep IgG antibody (1 in 2500 dilution) and enhanced chemiluminescence.