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Plasmid
pET6HIS SUMO PArkin 80-465 K211A
Parent Plasmid
pET15b 6His SUMO1
Expressed
His-SUMO-Parkin 80-465 K211A
DU Number
DU43842
Genbank
O60260
Tag
SUMO
6His
Species
Human
Sequence of Insert
View Sequence
CCATGGGTCATCATCACCATCACCATTCTGACCAGGAGGCAAAACCTTCA
ACTGAGGACTTGGGGGATAAGAAGGAAGGTGAATATATTAAACTCAAAGT
CATTGGACAGGATAGCAGTGAGATTCACTTCAAAGTGAAAATGACAACAC
ATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTGTTCCAATG
AACTCACTCAGGTTTCTCTTTGAGGGTCAGAGAATTGCTGATAATCATAC
TCCAAAAGAACTGGGAATGGAGGAAGAAGATGTGATTGAAGTTTATCAGG
AACAAACGGGGGGaatgaatgcaactggaggcgacgaccccagaaacgcg
gcgggaggctgtgagcgggagccccagagcttgactcgggtggacctcag
cagctcagtcctcccaggagactctgtggggctggctgtcattctgcaca
ctgacagcaggaaggactcaccaccagctggaagtccagcaggtagatca
atctacaacagcttttatgtgtattgcaaaggcccctgtcaaagagtgca
gccgggaaaactcagggtacagtgcagcacctgcaggcaggcaacgctca
ccttgacccagggtccatcttgctgggatgatgttttaattccaaaccgg
atgagtggtgaatgccaatccccacactgccctgggactagtgcagaatt
tttctttGCatgtggagcacaccccacctctgacaaggaaacatcagtag
ctttgcacctgatcgcaacaaatagtcggaacatcacttgcattacgtgc
acagacgtcaggagccccgtcctggttttccagtgcaactcccgccacgt
gatttgcttagactgtttccacttatactgtgtgacaagactcaatgatc
ggcagtttgttcacgaccctcaacttggctactccctgccttgtgtggct
ggctgtcccaactccttgattaaagagctccatcacttcaggattctggg
agaagagcagtacaaccggtaccagcagtatggtgcagaggagtgtgtcc
tgcagatggggggcgtgttatgcccccgccctggctgtggagcggggctg
ctgccggagcctgaccagaggaaagtcacctgcgaagggggcaatggcct
gggctgtgggtttgccttctgccgggaatgtaaagaagcgtaccatgaag
gggagtgcagtgccgtatttgaagcctcaggaacaactactcaggcctac
agagtcgatgaaagagccgccgagcaggctcgttgggaagcagcctccaa
agaaaccatcaagaaaaccaccaagccctgtccccgctgccatgtaccag
tggaaaaaaatggaggctgcatgcacatgaagtgtccgcagccccagtgc
aggctcgagtggtgctggaactgtggctgcgagtggaaccgcgtctgcat
gggggaccactggttcgacgtgtaggcggccgc
ACTGAGGACTTGGGGGATAAGAAGGAAGGTGAATATATTAAACTCAAAGT
CATTGGACAGGATAGCAGTGAGATTCACTTCAAAGTGAAAATGACAACAC
ATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTGTTCCAATG
AACTCACTCAGGTTTCTCTTTGAGGGTCAGAGAATTGCTGATAATCATAC
TCCAAAAGAACTGGGAATGGAGGAAGAAGATGTGATTGAAGTTTATCAGG
AACAAACGGGGGGaatgaatgcaactggaggcgacgaccccagaaacgcg
gcgggaggctgtgagcgggagccccagagcttgactcgggtggacctcag
cagctcagtcctcccaggagactctgtggggctggctgtcattctgcaca
ctgacagcaggaaggactcaccaccagctggaagtccagcaggtagatca
atctacaacagcttttatgtgtattgcaaaggcccctgtcaaagagtgca
gccgggaaaactcagggtacagtgcagcacctgcaggcaggcaacgctca
ccttgacccagggtccatcttgctgggatgatgttttaattccaaaccgg
atgagtggtgaatgccaatccccacactgccctgggactagtgcagaatt
tttctttGCatgtggagcacaccccacctctgacaaggaaacatcagtag
ctttgcacctgatcgcaacaaatagtcggaacatcacttgcattacgtgc
acagacgtcaggagccccgtcctggttttccagtgcaactcccgccacgt
gatttgcttagactgtttccacttatactgtgtgacaagactcaatgatc
ggcagtttgttcacgaccctcaacttggctactccctgccttgtgtggct
ggctgtcccaactccttgattaaagagctccatcacttcaggattctggg
agaagagcagtacaaccggtaccagcagtatggtgcagaggagtgtgtcc
tgcagatggggggcgtgttatgcccccgccctggctgtggagcggggctg
ctgccggagcctgaccagaggaaagtcacctgcgaagggggcaatggcct
gggctgtgggtttgccttctgccgggaatgtaaagaagcgtaccatgaag
gggagtgcagtgccgtatttgaagcctcaggaacaactactcaggcctac
agagtcgatgaaagagccgccgagcaggctcgttgggaagcagcctccaa
agaaaccatcaagaaaaccaccaagccctgtccccgctgccatgtaccag
tggaaaaaaatggaggctgcatgcacatgaagtgtccgcagccccagtgc
aggctcgagtggtgctggaactgtggctgcgagtggaaccgcgtctgcat
gggggaccactggttcgacgtgtaggcggccgc
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMNATGGDDPRNAAGGCEREPQSLTRVDLSSSVLPGDSVGLAVILHT
DSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGKLRVQCSTCRQATLT
LTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFACGAHPTSDKETSVA
LHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFHLYCVTRLNDR
QFVHDPQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRYQQYGAEECVL
QMGGVLCPRPGCGAGLLPEPDQRKVTCEGGNGLGCGFAFCRECKEAYHEG
ECSAVFEASGTTTQAYRVDERAAEQARWEAASKETIKKTTKPCPRCHVPV
EKNGGCMHMKCPQPQCRLEWCWNCGCEWNRVCMGDHWFDV*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMNATGGDDPRNAAGGCEREPQSLTRVDLSSSVLPGDSVGLAVILHT
DSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGKLRVQCSTCRQATLT
LTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFACGAHPTSDKETSVA
LHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFHLYCVTRLNDR
QFVHDPQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRYQQYGAEECVL
QMGGVLCPRPGCGAGLLPEPDQRKVTCEGGNGLGCGFAFCRECKEAYHEG
ECSAVFEASGTTTQAYRVDERAAEQARWEAASKETIKKTTKPCPRCHVPV
EKNGGCMHMKCPQPQCRLEWCWNCGCEWNRVCMGDHWFDV*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Introduce K211A mutation into clone DU39813
100042 R 1000 1001 1047 19007
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