parkin Ser223Pro Polymorphic Varian

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Synonyms

park2

Plasmid

pET6HIS SUMO parkin I44A

Parent Plasmid

pET15b 6His SUMO1

Expressed

6HIS SUMO parkin I44A S223P

DU Number

DU32839

Genbank

AAN75850.1

Tag

SUMO

Species

Human

Sequence of Insert

View Sequence

GGATCCATGAACCCCGGCTTCGATTTGTCCCGCCGGAACCCGCAGGAGGA
CTTCGAGCTGATTCAGCGCATCGGCAGCGGCACCTACGGCGACGTCTACA
AGGCACGGAATGTTAACACTGGTGAATTAGCAGCAATTAAAGTAATAAAA
TTGGAACCAGGAGAAGACTTTGCAGTTGTGCAGCAAGAAATTATCATGAT
GAAAGACTGTAAACACCCAAATATTGTTGCTTATTTTGGAAGCTATCTCA
GGCGAGATAAGCTTTGGATTTGCATGGAGTTTTGTGGAGGTGGTTCTTTA
CAGGATATTTATCACGTAACTGGACCTCTGTCAGAACTGCAAATTGCATA
TGTTAGCAGAGAAACACTGCAGGGATTATATTATCTTCACAGTAAAGGAA
AAATGCACAGAGATATAAAGGGAGCTAACATTCTATTAACGGATAATGGT
CATGTGAAATTGGCTGATTTTGGAGTATCTGCACAGATAACAGCTACAAT
TGCCAAACGGAAGTCTTTCATTGGCACACCATATTGGATGGCTCCAGAAG
TTGCAGCTGTTGAGAGGAAGGGGGGTTACAATCAACTCTGTGATCTCTGG
GCAGTGGGAATCACTGCCATAGAACTTGCAGAGCTTCAGCCTCCTATGTT
TGACTTACACCCAATGAGAGCATTATTTCTAATGACAAAAAGCAATTTTC
AGCCTCCTAAACTAAAGGATAAAATGAAATGGTCAAATAGTTTTCATCAC
TTTGTGAAAATGGCACTTACCAAAAATCCGAAAAAAAGACCTACTGCTGA
AAAATTATTACAGCATCCTTTTGTAACACAACATTTGACACGGTCTTTGG
CAATCGAGCTGTTGGATAAAGTAAATAATCCAGATCATTCCACTTACCAT
GATTTCGATGATGATGATCCTGAGCCTCTTGTTGCTGTACCACATAGAAT
TCACTCAACAAGTAGAAACGTGAGAGAAGAAAAAACACGCTCAGAGATAA
CCTTTGGCCAAGTGAAATTTGATCCACCCTTAAGAAAGGAGACAGAACCA
CATCATGAACTTGATCTGCAACTGGAATATGGACAAGGACACCAAGGTGG
TTACTTTTTAGGTGCAAACAAGAGTCTTCTCAAGTCTGTTGAAGAAGAAT
TGCATCAGCGAGGACACGTCGCACATTTAGAAGATGATGAAGGAGATGAT
GATGAATCTAAACACTCAACTCTGAAAGCAAAAATTCCACCTCCTTTGCC
ACCAAAGCCTAAGTCTATCTTCATACCACAGGAAATGCATTCTACTGAGG
ATGAAAATCAAGGAACAATCAAGAGATGTCCCATGTCAGGGAGCCCAGCA
AAGCCATCCCAAGTTCCACCTAGACCACCACCTCCCAGATTACCCCCACA
CAAACCTGTTGCCTTAGGAAATGGAATGAGCTCCTTCCAGTTAAATGGTG
AACGAGATGGCTCATTATGTCAACAACAGAATGAACATAGAGGCACAAAC
CTTTCAAGAAAAGAAAAGAAAGATGTACCAAAGCCTATTAGTAATGGTCT
TCCTCCAACACCTAAAGTGCATATGGGTGCATGTTTTTCAAAAGTTTTTA
ATGGGTGTCCCTTGAAAATTCACTGTGCATCATCATGGATAAACCCAGAT
ACTAGAGATCAGTACTTGATATTTGGTGCCGAAGAAGGGATTTATACCCT
CAATCTTAATGAACTTCATGAAACATCAATGGAACAGCTATTCCCTCGAA
GGTGTACATGGTTGTATGTAATGAACAATTGCTTGCTATCAATATCTGGT
AAAGCTTCTCAGCTTTATTCCCATAATTTACCAGGGCTTTTTGATTATGC
AAGACAAATGCAAAAGTTACCTGTTGCTATTCCAGCACACAAACTCCCTG
ACAGAATACTGCCAAGGAAATTTTCTGTATCAGCAAAAATCCCTGAAACC
AAATGGTGCCAGAAGTGTTGTGTTGTAAGAAATCCTTACACGGGCCATAA
ATACCTATGTGGAGCACTTCAGACTAGCATTGTTCTATTAGAATGGGTTG
AACCAATGCAGAAATTTATGTTAATTAAGCACATAGATTTTCCTATACCA
TGTCCACTTAGAATGTTTGAAATGCTGGTAGTTCCTGAACAGGAGTACCC
TTTAGTTTGTGTTGGTGTCAGTAGAGGTAGAGACTTCAACCAAGTGGTTC
GATTTGAGACGGTCAATCCAAATTCTACCTCTTCATGGTTTACAGAATCA
GATACCCCACAGACAAATGTTACTCATGTAACCCAACTGGAGAGAGATAC
CATCCTTGTATGCTTGGACTGTTGTATAAAAATAGTAAATCTCCAAGGAA
GATTAAAATCTAGCAGGAAATTGTCATCAGAACTCACCTTTGATTTCCAG
ATTGAATCAATAGTGTGCCTACAAGACAGTGTGCTAGCTTTCTGGAAACA
TGGAATGCAAGGTAGAAGTTTTAGATCTAATGAGGTAACACAAGAAATTT
CAGATAGCACAAGAATTTTCAGGCTGCTTGGATCTGACAGGGTCGTGGTT
TTGGAAAGTAGGCCAACTGATAACCCCACAGCAAATAGCAATTTGTACAT
CCTGGCGGGTCATGAAAACAGTTACTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMNPGFDLSRRNPQEDFELIQRI
GSGTYGDVYKARNVNTGELAAIKVIKLEPGEDFAVVQQEIIMMKDCKHPN
IVAYFGSYLRRDKLWICMEFCGGGSLQDIYHVTGPLSELQIAYVSRETLQ
GLYYLHSKGKMHRDIKGANILLTDNGHVKLADFGVSAQITATIAKRKSFI
GTPYWMAPEVAAVERKGGYNQLCDLWAVGITAIELAELQPPMFDLHPMRA
LFLMTKSNFQPPKLKDKMKWSNSFHHFVKMALTKNPKKRPTAEKLLQHPF
VTQHLTRSLAIELLDKVNNPDHSTYHDFDDDDPEPLVAVPHRIHSTSRNV
REEKTRSEITFGQVKFDPPLRKETEPHHELDLQLEYGQGHQGGYFLGANK
SLLKSVEEELHQRGHVAHLEDDEGDDDESKHSTLKAKIPPPLPPKPKSIF
IPQEMHSTEDENQGTIKRCPMSGSPAKPSQVPPRPPPPRLPPHKPVALGN
GMSSFQLNGERDGSLCQQQNEHRGTNLSRKEKKDVPKPISNGLPPTPKVH
MGACFSKVFNGCPLKIHCASSWINPDTRDQYLIFGAEEGIYTLNLNELHE
TSMEQLFPRRCTWLYVMNNCLLSISGKASQLYSHNLPGLFDYARQMQKLP
VAIPAHKLPDRILPRKFSVSAKIPETKWCQKCCVVRNPYTGHKYLCGALQ
TSIVLLEWVEPMQKFMLIKHIDFPIPCPLRMFEMLVVPEQEYPLVCVGVS
RGRDFNQVVRFETVNPNSTSSWFTESDTPQTNVTHVTQLERDTILVCLDC
CIKIVNLQGRLKSSRKLSSELTFDFQIESIVCLQDSVLAFWKHGMQGRSF
RSNEVTQEISDSTRIFRLLGSDRVVVLESRPTDNPTANSNLYILAGHENS
Y*

Vector Type

Bacterial

Antibiotic

Amp

Comments

N ter 6HIS for baculovirus expression

Estimated MW of expressed protein : 102324 Daltons

100042 R 1000 1001 1047 19007

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