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PINK1 (Trichoplax adherens)

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Plasmid
pET6HIS SUMO Pink1 (trichoplax) D358A
Parent Plasmid
pET6HIS SUMO
Expressed
6HIS SUMO pink1 trichoplax D358A
DU Number
DU51015
Genbank
XP_002109370.1
Tag
6His
SUMO
Species
Trichoplax adhaerens
Sequence of Insert
GGAATGGCTCTGATTCTGCGTCGTATCTTTAACAACCTGTCGAAATTCCTGCCGCAATTTGAACCGGGCTTTGAACGTGGTGCGAAACAAGTTTTTACCG
AAAACATTAACAATGCAACGCTGACCACGCGCATTGTCAGCAAAGATAACCAGATCGTGGTTAAAAACTCGAACTACAGCAAACTGGAAAATGGCTCCGT
CTCACGTCTGCGTATCGTGGCACGTGGCCTGATTGGTAAATCTTACATCAGTAACAATCTGCACCTGATTAAGAAAATTGCATCGGCTACCCGTCTGGTT
TTTACGCGTCGCTTCCCGCTGAGCACCCCGATTTTTGCACTGCTGGGTTTCACGGTGGCAGGTCCGCGTTATAACGAACAGCAAATGGAACAGCAAAAAC
TGGTCGTGTCCCTGCAGTCACTGCTGAAAATCTGCCATAACAAACTGGAAAAAGATAATGCGGCCTCCGAAATCTCGTGCAGCTGTGACACCACGAATTC
ATATAAAGTCGGCCCGCGTCTGGGTTCTCGCAGTTGCTGTTCTGCGGTGTACGCCACCAAATGCAGTTGTGGCAAAGAATACGCGATGAAAATGATGTTC
AACTACGGTAACTCTAAAGATGACTTCATCCGCAAAAACTTCCTGCGTGAATATTGCCTGCTGAGTCCGCTGCCGGTGACGCGTGTTGATGAAATGGAAA
GCTCTAAAAACTCCCAGTCACGCATTCTGGTGCACCCGAACATCATCCGTCTGGATAAAGTTATCATCGACGAAATCCAATACTGTGATGACGCAATGCA
TGAATACCCGGATGCTCTGCCGCCGTCGCCGCTGAATCCGAGCGGTCTGGGTCGTCACCGTACCATGTTCCTGTTCATGAAAAAATACGACATGACGCTG
CGCGAATACGTTCATTCCAAAGGCATTATCCCGAACGATGAAGCAACCATGCTGCTGCTGCAGCTGCTGCAGGGTGTGGACTATCTGAACAAAAATTACA
TTGCTCACCGTGATCTGAAAAGCGACAATATTCTGATCGAAGAAGTTGATCCGTATCCGCTGCTGGCGATCACCGcCTTCGGCGAATGCCTGGATGACGC
AGCTCTGAAACTGGTTGTCCCGTACATTAGCAACTGTATCGATCGCGGCGGTAATAGTTCCCTGATGGCACCGGAAATTAAAAACGCTGAACCGGGTCAT
AATTCTGTTCTGGATTATCGTCGCAGTGACGTCTGGGCGATTGGCTGCCTGGCCTTTGAAATCTTCGGTCAGGAAAACCCGTTTCTGAATACCTTCGATT
CATCGAGCTATCAAGAATACGAACTGCCGGAACTGCCGTCTCGTGTCCCGCTGATTGTGCGTAAAATCGTTCGCAACCTGCTGCGTAAAGATTCTAGTAA
TCGTCTGCCGGCATCCCTGGCTATTGCAGCACTGTCATTTTGGCGTTGGGGTCCGATCAATTGGCGTGCACTGATGATGTATGAAACCGGTCTGAGTAAC
CTGAATGAAGAAGATATTCAGCGTTGGCTGTCGGAAACGGCCTTCCAGCTGAGCATTCAAATCATGCAGTGTCAAGCGCATGGTCACAAACTGACCCCGG
AACTGCAGGCCAAATACCATTTTCTGGTCTCAACGAGTGCCGCTGACATCAAACTGGCTGTGGAATCCCTGTATTGCTAAGCGGCCGC
Amino Acid Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMALILRRIFNNLSKFLPQFEPGFERGAKQVFTENINNATLTTRIVSKDNQIVVKNSNYSKLENGSVSRLRIVARGLIGKSYISNNLHLIKKIASAT
RLVFTRRFPLSTPIFALLGFTVAGPRYNEQQMEQQKLVVSLQSLLKICHNKLEKDNAASEISCSCDTTNSYKVGPRLGSRSCCSAVYATKCSCGKEYAMK
MMFNYGNSKDDFIRKNFLREYCLLSPLPVTRVDEMESSKNSQSRILVHPNIIRLDKVIIDEIQYCDDAMHEYPDALPPSPLNPSGLGRHRTMFLFMKKYD
MTLREYVHSKGIIPNDEATMLLLQLLQGVDYLNKNYIAHRDLKSDNILIEEVDPYPLLAITAFGECLDDAALKLVVPYISNCIDRGGNSSLMAPEIKNAE
PGHNSVLDYRRSDVWAIGCLAFEIFGQENPFLNTFDSSSYQEYELPELPSRVPLIVRKIVRNLLRKDSSNRLPASLAIAALSFWRWGPINWRALMMYETG
LSNLNEEDIQRWLSETAFQLSIQIMQCQAHGHKLTPELQAKYHFLVSTSAADIKLAVESLYC*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Codon optimised for bacterial expression
100042 R 1000 1001 1047 19007
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