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Plasmid
pET6HIS SUMO Pink1 (anopheles) D466A
Parent Plasmid
pET 6HIS SUMO
Expressed
6HIS SUMO pink1 (anapheles) D466A
DU Number
DU51033
Genbank
XP_313587
Tag
6His
SUMO
Species
Anopheles gambiae
Sequence of Insert
View Sequence
ATGTCCTTTCGTCTGCTGACCACACGTTTGTACAAACATGGTCGCCTGCTGGTCCAGAACTATCTCAAGCGGGATATACACGTTAGCAACGTTTGGACCG
GCCCCACGGAGAGCACAAAAGTGAACAAGATTAACGAAACGCTGCGACATGATCTGCGGATCAACGATTTCCGGAACAACAGCTTCCTGCGGTTTGGCAA
CCAGGCCCGTCGTCTCTTTATCGACAATGTGCTAAACAGGGTCACCAACCCGTACTCGGTGGATCTACGACTACAGGCTACGAAAAAACTGTTGTACGGC
GATTCGACACCTTTCTTTGCCTTGGTAGGTGTGAGTTTGGCTTCCGGAGATGGTGTTCTGACTAAGAATGACGAGCTGGAAGCAGTCTGTTGGGAAATTA
GGCATGCAATGTCCAACTTTCAACAGAAAGTCGGCGAGAAGGACATCGAATCTCGACTGGACGAAGAGTTCGGCATTGAGAACTTAAACATTGGCAAACC
CATCGCCAAAGGCTGCTCCGCCGTTGTTTACGCAGCTTCTCTCAAAGAATCTACCGTAAGCGATAACGATGATATGAACTGCAATGATATTCATAACTCA
AAGTCCAGTTCGAATGGGAAATGTCCGAATGAAGCATGGACAGGAGATGAGCCGGAGCTTTCTGGACAGCGGAGTGACGGGATTAGTCCGCTGCTGCTTC
CCGAACGAACTGCATCGGAGGCTTTCCATCAAATGATGAACGAAAGCAATGCGCAAACGAGAACAAACAACAATCGTAAAGTTCGCTTTAATAGTGAAAC
TAGAATAAGAACAATGTCGGAAAACAGCGCCTCCAATCATGAAACCATTGGGCATTATCAGCCTGACTCAATCGAACAGGACTACACGATCGAAGAGTAT
CCACTGGCGTTAAAGATGATGTTTAATTACGACATACAAAGCAACGCCATGGCTATCCTAAAGGCGATGTACCGTGAAACTGTCCCAGCGAAGAGAAGAA
CTGTTGACAACGCGTGGGAAAAAAGCCTCATGGAAAAGACCAATTTTGTTCCACCCCATCCAAATATTGTGGAAATGTACGGCGTTTTCTGCGACCAGGT
ACCGGATTTGTCAATGTCGGCCACGCTGTACCCTATGGCATTGCCACAGCGGCTCAACCCGCAAGGCTACGGGCGAAACATGAGCCTTTTCCTTTTGATG
AAAAGATACAACATCAATCTGAAAGACTATCTGCGCCAACCGGGGGTCGATATGCGTACGCGCATTCTACTGTTTGCGCAGCTGCTGGAAGCCGTTGCTC
ATCTGAATCGTCACGGGGTCTCTCATCGGGATATAAAGTCGGACAACATACTGATCGAATTGCGGCCAAACATGCCACCGACGCTGGTGCTGACCGcCTT
TGGCTGCTGCATTGCGGACAAGCGCCATGGGCTGCGCATCCCTTACACTTCGGACGAGATCGATAAGGGTGGTAACGTGGCACTAATGGCGCCGGAGATA
ATAGAGCAGCTACCGGGAACGTTCGCCATGCTGAATTACACCAAAGCCGACCTGTGGGCATGTGGTGCAATCGCTTATGAAATATTTGGCAGCAATAATC
CTTTTTACTCCGACGTGAATTCGGCATTAAAGAACACGACGTATGAAGAAGATATGCTACCCGCGATGGATCAAAATGTCCCGAGGCTGATCCAATGTCT
TGTGCAGAACATACTGCAAAGAAACCCTAGTAAGCGCTTAAGCCCGGACATTGCAGCCAACGTGGTGCAATTGTTCTTGTGGTCACCATCATCGTGGTTA
CGCGATAGGTACGTACCGTCCAGCAATGAAATTCTGCAATGGCTGCTCAGCCTCACGACGAAAATACTGTGCGAAGGCCCGTTGCGCGTGACTCCCGACG
GTACTATGGGGCGCAGAACGTACACGGAGTACTTACTGATTGCAAGCTTTCTTACCCGGGTACGCTTGGAAAGGATCAAGCGCGCGCTCGATTGGATACA
TAATGTTAACGCTGGCTGTTCGTAAGCGGCCGC
GCCCCACGGAGAGCACAAAAGTGAACAAGATTAACGAAACGCTGCGACATGATCTGCGGATCAACGATTTCCGGAACAACAGCTTCCTGCGGTTTGGCAA
CCAGGCCCGTCGTCTCTTTATCGACAATGTGCTAAACAGGGTCACCAACCCGTACTCGGTGGATCTACGACTACAGGCTACGAAAAAACTGTTGTACGGC
GATTCGACACCTTTCTTTGCCTTGGTAGGTGTGAGTTTGGCTTCCGGAGATGGTGTTCTGACTAAGAATGACGAGCTGGAAGCAGTCTGTTGGGAAATTA
GGCATGCAATGTCCAACTTTCAACAGAAAGTCGGCGAGAAGGACATCGAATCTCGACTGGACGAAGAGTTCGGCATTGAGAACTTAAACATTGGCAAACC
CATCGCCAAAGGCTGCTCCGCCGTTGTTTACGCAGCTTCTCTCAAAGAATCTACCGTAAGCGATAACGATGATATGAACTGCAATGATATTCATAACTCA
AAGTCCAGTTCGAATGGGAAATGTCCGAATGAAGCATGGACAGGAGATGAGCCGGAGCTTTCTGGACAGCGGAGTGACGGGATTAGTCCGCTGCTGCTTC
CCGAACGAACTGCATCGGAGGCTTTCCATCAAATGATGAACGAAAGCAATGCGCAAACGAGAACAAACAACAATCGTAAAGTTCGCTTTAATAGTGAAAC
TAGAATAAGAACAATGTCGGAAAACAGCGCCTCCAATCATGAAACCATTGGGCATTATCAGCCTGACTCAATCGAACAGGACTACACGATCGAAGAGTAT
CCACTGGCGTTAAAGATGATGTTTAATTACGACATACAAAGCAACGCCATGGCTATCCTAAAGGCGATGTACCGTGAAACTGTCCCAGCGAAGAGAAGAA
CTGTTGACAACGCGTGGGAAAAAAGCCTCATGGAAAAGACCAATTTTGTTCCACCCCATCCAAATATTGTGGAAATGTACGGCGTTTTCTGCGACCAGGT
ACCGGATTTGTCAATGTCGGCCACGCTGTACCCTATGGCATTGCCACAGCGGCTCAACCCGCAAGGCTACGGGCGAAACATGAGCCTTTTCCTTTTGATG
AAAAGATACAACATCAATCTGAAAGACTATCTGCGCCAACCGGGGGTCGATATGCGTACGCGCATTCTACTGTTTGCGCAGCTGCTGGAAGCCGTTGCTC
ATCTGAATCGTCACGGGGTCTCTCATCGGGATATAAAGTCGGACAACATACTGATCGAATTGCGGCCAAACATGCCACCGACGCTGGTGCTGACCGcCTT
TGGCTGCTGCATTGCGGACAAGCGCCATGGGCTGCGCATCCCTTACACTTCGGACGAGATCGATAAGGGTGGTAACGTGGCACTAATGGCGCCGGAGATA
ATAGAGCAGCTACCGGGAACGTTCGCCATGCTGAATTACACCAAAGCCGACCTGTGGGCATGTGGTGCAATCGCTTATGAAATATTTGGCAGCAATAATC
CTTTTTACTCCGACGTGAATTCGGCATTAAAGAACACGACGTATGAAGAAGATATGCTACCCGCGATGGATCAAAATGTCCCGAGGCTGATCCAATGTCT
TGTGCAGAACATACTGCAAAGAAACCCTAGTAAGCGCTTAAGCCCGGACATTGCAGCCAACGTGGTGCAATTGTTCTTGTGGTCACCATCATCGTGGTTA
CGCGATAGGTACGTACCGTCCAGCAATGAAATTCTGCAATGGCTGCTCAGCCTCACGACGAAAATACTGTGCGAAGGCCCGTTGCGCGTGACTCCCGACG
GTACTATGGGGCGCAGAACGTACACGGAGTACTTACTGATTGCAAGCTTTCTTACCCGGGTACGCTTGGAAAGGATCAAGCGCGCGCTCGATTGGATACA
TAATGTTAACGCTGGCTGTTCGTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMSFRLLTTRLYKHGRLLVQNYLKRDIHVSNVWTGPTESTKVNKINETLRHDLRINDFRNNSFLRFGNQARRLFIDNVLNRVTNPYSVDLRLQATKK
LLYGDSTPFFALVGVSLASGDGVLTKNDELEAVCWEIRHAMSNFQQKVGEKDIESRLDEEFGIENLNIGKPIAKGCSAVVYAASLKESTVSDNDDMNCND
IHNSKSSSNGKCPNEAWTGDEPELSGQRSDGISPLLLPERTASEAFHQMMNESNAQTRTNNNRKVRFNSETRIRTMSENSASNHETIGHYQPDSIEQDYT
IEEYPLALKMMFNYDIQSNAMAILKAMYRETVPAKRRTVDNAWEKSLMEKTNFVPPHPNIVEMYGVFCDQVPDLSMSATLYPMALPQRLNPQGYGRNMSL
FLLMKRYNINLKDYLRQPGVDMRTRILLFAQLLEAVAHLNRHGVSHRDIKSDNILIELRPNMPPTLVLTAFGCCIADKRHGLRIPYTSDEIDKGGNVALM
APEIIEQLPGTFAMLNYTKADLWACGAIAYEIFGSNNPFYSDVNSALKNTTYEEDMLPAMDQNVPRLIQCLVQNILQRNPSKRLSPDIAANVVQLFLWSP
SSWLRDRYVPSSNEILQWLLSLTTKILCEGPLRVTPDGTMGRRTYTEYLLIASFLTRVRLERIKRALDWIHNVNAGCS*
QTGGMSFRLLTTRLYKHGRLLVQNYLKRDIHVSNVWTGPTESTKVNKINETLRHDLRINDFRNNSFLRFGNQARRLFIDNVLNRVTNPYSVDLRLQATKK
LLYGDSTPFFALVGVSLASGDGVLTKNDELEAVCWEIRHAMSNFQQKVGEKDIESRLDEEFGIENLNIGKPIAKGCSAVVYAASLKESTVSDNDDMNCND
IHNSKSSSNGKCPNEAWTGDEPELSGQRSDGISPLLLPERTASEAFHQMMNESNAQTRTNNNRKVRFNSETRIRTMSENSASNHETIGHYQPDSIEQDYT
IEEYPLALKMMFNYDIQSNAMAILKAMYRETVPAKRRTVDNAWEKSLMEKTNFVPPHPNIVEMYGVFCDQVPDLSMSATLYPMALPQRLNPQGYGRNMSL
FLLMKRYNINLKDYLRQPGVDMRTRILLFAQLLEAVAHLNRHGVSHRDIKSDNILIELRPNMPPTLVLTAFGCCIADKRHGLRIPYTSDEIDKGGNVALM
APEIIEQLPGTFAMLNYTKADLWACGAIAYEIFGSNNPFYSDVNSALKNTTYEEDMLPAMDQNVPRLIQCLVQNILQRNPSKRLSPDIAANVVQLFLWSP
SSWLRDRYVPSSNEILQWLLSLTTKILCEGPLRVTPDGTMGRRTYTEYLLIASFLTRVRLERIKRALDWIHNVNAGCS*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Codon optimised for bacterial expression
100042 R 1000 1001 1047 19007
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