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Plasmid
pET15b 6HIS SUMO Tribolium PINK1 E125-end D359A E527A K528A
Parent Plasmid
pET15b 6His SUMO
Expressed
6HIS SUMO Tribolium PINK1 E125-end D359A E527A K528A
DU Number
DU26015
Genbank
XP_968367 (codon-optimised)
Tag
6His
SUMO
Species
Tribolium castaneum
Sequence of Insert
View Sequence
GAACTGGAAGGCGTTTGCTGGGAAATCCGTGAAGCGATCTCTAAAATCAAATGGCAGTACTACGATATCGACGAAAGCCGCTTTGAATCTAACCCGATCACCCTGAATGATCTGAGTCTGGGTAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAGATGACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATGTTCAACTACGATATTCAGAGCAATTCTATGGAAATCCTGAAAGCTATGTATCGTGAAACCGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACAATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAATATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGAAGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAGGTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGATTGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCTGCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACGGTATTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAACCGGAATCGCCGATTCTGGTTATCTCGGCTTTTGGCTGCTGTCTGGCGGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAGGCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCGGGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGCAATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGTCTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTGCCGAAACTGCCGGACGAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAACGTAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGTTTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCGACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTGCGAAGGCAAAATCAACAACAAATCCTTCGGTGCAGCATTCACCCGTAATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGCGCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAACCTGCCGGAACTGGATTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGELEGVCWEIREAISKIKWQYYDIDESRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLPPHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLDTSEPESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPKLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGAAFTRNWRRTYPEYLLISSFLCRAKLANVRNALHWIQENLPELD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Linker after SENP cleavage deleted
100042 R 1000 1001 1047 19007
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