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Plasmid
pMAL4C Pink1 123-581 T185E S187E H271Q T257E S284E
Parent Plasmid
pMAL4C
Expressed
MBP Pink1 123-581 T185E S187E H271Q T257E S284E
DU Number
DU46381
Genbank
Q9BXM7
Tag
MBP
Sequence of Insert
View Sequence
CTCGGCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAAAAGCAAGCCGG
GGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTAGGCTGGAGGAG
TATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCTGTGTATGA
AGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGGAAAAGGAAACCG
GGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAGGGCAG
GAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGATGATGTG
GAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAATGAGCC
AGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATGGAGCA
GTCGAATACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCCCCTCAaCC
CAACATCATCCGGGTTCTCCGCGCCTTCACCTCTGAAGTGCCGCTGCTGC
CAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCCTCCACCCT
GAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAACTATCC
CTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCGCCTCG
CCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGGTTCAA
CAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCTTGTGGAGCT
GGACCCAGACGGCTGCCCCTGGCTGGTGAtcgcagattttggctgctgcc
tggctgatgagagcatcggcctgcagttgcccttcagcagctggtacgtg
gatcggggcggaaacggctgtctgatggccccagaggtgtccacggcccg
tcctggccccagggcagtgattgactacagcaaggctgatgcctgggcag
tgggagccatcgcctatgaaatcttcgggcttgtcaatcccttctacggc
cagggcaaggcccaccttgaaagccgcagctaccaagaggctcagctacc
tgcactgcccgagtcagtgcctccagacgtgagacagttggtgagggcac
tgctccagcgagaggccagcaagagaccatctgcccgagtagccgcaaat
gtgcttcatctaagcctctggggtgaacatattctagccctgaagaatct
gaagttagacaagatggttggctggctcctccaacaatcggccgccactt
tgttggccaacaggctcacagagaagtgttgtgtggaaacaaaaatgaag
atgctctttctggctaacctggagtgtgaaacgctctgccaggcagccct
cctcctctgctcatggagggcagccctgtgagcggccgc
GGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTAGGCTGGAGGAG
TATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCTGTGTATGA
AGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGGAAAAGGAAACCG
GGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAGGGCAG
GAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGATGATGTG
GAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAATGAGCC
AGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATGGAGCA
GTCGAATACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCCCCTCAaCC
CAACATCATCCGGGTTCTCCGCGCCTTCACCTCTGAAGTGCCGCTGCTGC
CAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCCTCCACCCT
GAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAACTATCC
CTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCGCCTCG
CCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGGTTCAA
CAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCTTGTGGAGCT
GGACCCAGACGGCTGCCCCTGGCTGGTGAtcgcagattttggctgctgcc
tggctgatgagagcatcggcctgcagttgcccttcagcagctggtacgtg
gatcggggcggaaacggctgtctgatggccccagaggtgtccacggcccg
tcctggccccagggcagtgattgactacagcaaggctgatgcctgggcag
tgggagccatcgcctatgaaatcttcgggcttgtcaatcccttctacggc
cagggcaaggcccaccttgaaagccgcagctaccaagaggctcagctacc
tgcactgcccgagtcagtgcctccagacgtgagacagttggtgagggcac
tgctccagcgagaggccagcaagagaccatctgcccgagtagccgcaaat
gtgcttcatctaagcctctggggtgaacatattctagccctgaagaatct
gaagttagacaagatggttggctggctcctccaacaatcggccgccactt
tgttggccaacaggctcacagagaagtgttgtgtggaaacaaaaatgaag
atgctctttctggctaacctggagtgtgaaacgctctgccaggcagccct
cctcctctgctcatggagggcagccctgtgagcggccgc
Amino Acid Sequence
View Sequence
MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQ
VAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMF
NLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPT
FKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVIN
AASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
GSATSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSA
AVYEATMPTLPQNLEVEKETGLLPGRGPGTSAPGEGQERAPGAPAFPLAI
KMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVEYRKSKRGPKQL
APQPNIIRVLRAFTSEVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVM
KNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNI
LVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEV
STARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQE
AQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILA
LKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLC
QAALLLCSWRAAL*
VAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMF
NLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPT
FKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVIN
AASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
GSATSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSA
AVYEATMPTLPQNLEVEKETGLLPGRGPGTSAPGEGQERAPGAPAFPLAI
KMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVEYRKSKRGPKQL
APQPNIIRVLRAFTSEVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVM
KNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNI
LVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEV
STARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQE
AQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILA
LKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLC
QAALLLCSWRAAL*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Please introduce the following mutations: H271Q into the existing clone DU43815 (MBP-human PINK1 T185E/S187E/T257E/S284E (residues 123-end) in pMAL4C vector)
100042 R 1000 1001 1047 19007
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