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Plasmid
pMAL4C Pink1 123-581 T185E S187E T257E S284E S419P
Parent Plasmid
pMAL4C
Expressed
MBP Pink1 123-581 T185E S187E T257E S284E S419P
DU Number
DU46386
Genbank
Q9BXM7
Sequence of Insert
View Sequence
GGATCCGCCACCTCGGCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAA
AAGCAAGCCGGGGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTA
GGCTGGAGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCT
GCTGTGTATGAAGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGGA
AAAGGAAACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAG
GAGAAGGGCAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATC
AAGATGATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAA
CACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGG
AGTATGGAGCAGTCGAATACAGAAAATCCAAGAGAGGTCCCAAGCAACTA
GCCCCTCACCCCAACATCATCCGGGTTCTCCGCGCCTTCACCTCTGAAGT
GCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCAC
GCCTCCACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATG
AAGAACTATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAG
CCCCCGCCTCGCCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACC
ATCTGGTTCAACAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATC
CTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGATTT
TGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCA
GCTGGTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTG
CCCACGGCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGA
TGCCTGGGCAGTGGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATC
CCTTCTACGGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAG
GCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTT
GGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAG
TAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCC
CTGAAGAATCTGAAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATC
GGCCGCCACTTTGTTGGCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAA
CAAAAATGAAGATGCTCTTTCTGGCTAACCTGGAGTGTGAAACGCTCTGC
CAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAGCCCTGTGATACAAGGA
CGACGATGACAAGTAGCGGCCGC
AAGCAAGCCGGGGCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTA
GGCTGGAGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCT
GCTGTGTATGAAGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGGA
AAAGGAAACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAG
GAGAAGGGCAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATC
AAGATGATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAA
CACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGG
AGTATGGAGCAGTCGAATACAGAAAATCCAAGAGAGGTCCCAAGCAACTA
GCCCCTCACCCCAACATCATCCGGGTTCTCCGCGCCTTCACCTCTGAAGT
GCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCAC
GCCTCCACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATG
AAGAACTATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAG
CCCCCGCCTCGCCGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACC
ATCTGGTTCAACAGGGCATCGCGCACAGAGACCTGAAATCCGACAACATC
CTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGATTT
TGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCA
GCTGGTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTG
CCCACGGCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGA
TGCCTGGGCAGTGGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATC
CCTTCTACGGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAG
GCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTT
GGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAG
TAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCC
CTGAAGAATCTGAAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATC
GGCCGCCACTTTGTTGGCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAA
CAAAAATGAAGATGCTCTTTCTGGCTAACCTGGAGTGTGAAACGCTCTGC
CAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAGCCCTGTGATACAAGGA
CGACGATGACAAGTAGCGGCCGC
Amino Acid Sequence
View Sequence
MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQ
VAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMF
NLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPT
FKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVIN
AASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
GSATSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSA
AVYEATMPTLPQNLEVEKETGLLPGRGPGTSAPGEGQERAPGAPAFPLAI
KMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVEYRKSKRGPKQL
APHPNIIRVLRAFTSEVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVM
KNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNI
LVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEV
PTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQE
AQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILA
LKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLC
QAALLLCSWRAAL*
VAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRY
NGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMF
NLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLI
KNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPT
FKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPL
GAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVIN
AASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGP
GSATSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSA
AVYEATMPTLPQNLEVEKETGLLPGRGPGTSAPGEGQERAPGAPAFPLAI
KMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVEYRKSKRGPKQL
APHPNIIRVLRAFTSEVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVM
KNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNI
LVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEV
PTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQE
AQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILA
LKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLC
QAALLLCSWRAAL*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Please introduce the following mutations: S419P into the existing clone DU43815 (MBP-human PINK1 T185E/S187E/T257E/S284E (residues 123-end) in pMAL4C vector)
100042 R 1000 1001 1047 19007
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