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Plasmid
pET6HIS SUMO Pink1 Tribolium 150-570 D359A Q396A K397A
Parent Plasmid
pET15b 6His SUMO1
Expressed
6HIS SUMO Pink1 Tribolium 150-570 D359A Q396A K397A
DU Number
DU46261
Genbank
XP_968367.1
Tag
6His
SUMO
Species
Tribolium castaneum
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFAL
KMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLP
PHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMK
RYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLL
DTSEPESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIIC
AAPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLP
KLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGL
KVPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLISS
FLCRAKLANVRNALHWIQENLPELD*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFAL
KMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLP
PHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMK
RYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLL
DTSEPESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIIC
AAPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLP
KLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGL
KVPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLISS
FLCRAKLANVRNALHWIQENLPELD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Addition of two mutations QK (396-397) to the construct DU43181
100042 R 1000 1001 1047 19007
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