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Synonyms
PARK6
Plasmid
pET6HIS SUMO Pink1 tribolium 150-570 D359A K440A E441A
Parent Plasmid
pET15b 6His SUMO1
Expressed
6HIS SUMO Pink1 tribolium 150-570 D359A K440A E441A
DU Number
DU46262
Genbank
XP_968367.1
Tag
6His
SUMO
Species
Tribolium castaneum
Sequence of Insert
View Sequence
AGCCGCTTTGAATCTAACCCGATCACCCTGAATGATCTGAGTCTGGGTAA
ACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAG
ATGACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATGTTC
AACTACGATATTCAGAGCAATTCTATGGAAATCCTGAAAGCTATGTATCG
TGAAACCGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACAATT
GGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAAT
ATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGAAGG
TTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAG
GTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGATTGT
AATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCT
GCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACGGTA
TTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAA
CCGGAATCGCCGATTCTGGTTATCTCGGCTTTTGGCTGCTGTCTGGCGGA
CAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAG
GCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCGGGC
ACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGC
AATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGTCTC
GCCTGAAAAACTTCAACTACGCAGCAGGTGATCTGCCGAAACTGCCGGAC
GAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAACGTAA
CCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGT
TTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCGACG
TCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTG
CGAAGGCAAAATCAACAACAAATCCTTCGGTGAAAAATTCACCCGTAATT
GGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGC
GCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAACCT
GCCGGAACTGGATTAAGCGGCCGC
ACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAG
ATGACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATGTTC
AACTACGATATTCAGAGCAATTCTATGGAAATCCTGAAAGCTATGTATCG
TGAAACCGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACAATT
GGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAAT
ATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGAAGG
TTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAG
GTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGATTGT
AATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCT
GCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACGGTA
TTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAA
CCGGAATCGCCGATTCTGGTTATCTCGGCTTTTGGCTGCTGTCTGGCGGA
CAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAG
GCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCGGGC
ACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGC
AATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGTCTC
GCCTGAAAAACTTCAACTACGCAGCAGGTGATCTGCCGAAACTGCCGGAC
GAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAACGTAA
CCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGT
TTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCGACG
TCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTG
CGAAGGCAAAATCAACAACAAATCCTTCGGTGAAAAATTCACCCGTAATT
GGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGC
GCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAACCT
GCCGGAACTGGATTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFAL
KMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLP
PHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMK
RYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLL
DTSEPESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIIC
QKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYAAGDLP
KLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGL
KVPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLISS
FLCRAKLANVRNALHWIQENLPELD*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFAL
KMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLP
PHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMK
RYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLL
DTSEPESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIIC
QKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYAAGDLP
KLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGL
KVPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLISS
FLCRAKLANVRNALHWIQENLPELD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Addition of two mutations KE (440-441) to the construct DU43181
100042 R 1000 1001 1047 19007
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