PINK1 (C.elegans) | LRRK2 Resource

Plasmid

pET6HIS SUMO Pink1 C elegans 129-641 D361A

Parent Plasmid

pET15b 6His SUMO1

Expressed

6HIS SUMO Pink1 c elegans129-641 D361A

DU Number

DU46194

Genbank

NP_495017

Tag

6His

SUMO

Species

Caenorhabditis elegans

Sequence of Insert

View Sequence

ggaGAATGGCCGAATAGAATTGATTCTTATGAGTTTGGGGAATTTCTCGG
TCAAGGATGCAATGCAGCAGTTTACTCTGCGAGATTAGCCAATTCTGATG
CAGAATCCTCAGGGAATACTCACTATGGTGCAGGGTTTAATGAAGTCACA
AATATACTTGCAGAAATTCCGCCAGTTAGCAAAGTTGCACAAAAGAAATT
CCCGTTGGCAATCAAATTAATGTTTAATTTTGAACATGATCGCGATGGAG
ATGCTCATCTCTTGAAATCAATGGGAAATGAATTGGCTCCATATCCGAAT
GCTGCAAAGTTGCTCAATGGACAAATGGGAACATTTAGACCTCTTCCAGC
AAAACATCCAAATGTTGTTCGAATTCAGACAGCTTTTATTGATTCGTTAA
AAGTTTTGCCAGATGCGATTGAACGATATCCAGATGCCCTTCACACTGCA
CGTTGGTATGAGTCAATTGCCTCCGAACCGAAAACAATGTACGTAGTAAT
GAGACGATACCGACAAACACTTCATGAATATGTATGGACTCGTCATCGAA
ATTATTGGACAGGACGAGTGATAATTGCTCAACTATTAGAAGCATGTACA
TATCTTCATAAGCATAAAGTTGCTCAGCGAGACATGAAAAGTGATAATAT
TCTTCTGGAATATGATTTTGACGACGAGATTCCCCAATTAGTTGTCGCCG
CTTTTGGATGTGCACTTGCATGTGACAATTGGCAAGTAGACTATGAATCA
GATGAAGTTAGTCTTGGAGGAAATGCCAAGACAAAAGCACCAGAAATTGC
GACGGCTGTTCCTGGAAAGAATGTAAAAGTAAACTTCGAAATGGCAGATA
CATGGGCAGCTGGAGGCCTTTCTTATGAAGTTCTAACACGATCAAATCCA
TTCTACAAACTTCTTGATACTGCAACATACCAGGAATCAGAACTACCAGC
ACTCCCATCTCGTGTCAATTTTGTGGCACGAGATGTCATTTTTGACCTAC
TCAAGCGAGATCCTAATGAAAGAGTCAAGCCGAATATTGCTGCAAATGCG
TTGAATTTGTCATTGTTCAGAATGGGAGAAGATGTGAAGCAGATGATGGA
AAAATGTGGAATATCTCAAATGACTACTCTATTGGCTGGAAGTTCTAAAG
TTTTGAGTCAAAAAATCAATAGTCGTCTGGACAAAGTGATGAATCTGATT
ACTGCTGAAACTATCATGGCCAACCTAGCTCCACATTTGATTAGTCGAGC
AGAACGACAACTTCGAGCAACATTTCTTTCAAGAATGAATCGAGAAGATA
TTTGGAGAAGTCTTCAATATTTCTTCCCAGCTGGTGTTCAACTTGACACA
CCTGCCACATCATCAGACTGTTTGGAGACTATTTCCAGTTTGATGTCGAG
TTTTTCAAATGATTCAGAAAATTACGAGAAGCAACAGAAACCGGCTAAAA
ATGGATACAACAATGTTCCACTTCTTCTCAGAAATGTTATCCGTACAGAT
GCGGATGGAATCAATGGAATTGTACATAGAGTTCGATCTAAATAGgcggc
cgc

Amino Acid Sequence

View Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHSPKELGMEEEDVIEVYQE
QTGGEWPNRIDSYEFGEFLGQGCNAAVYSARLANSDAESSGNTHYGAGFN
EVTNILAEIPPVSKVAQKKFPLAIKLMFNFEHDRDGDAHLLKSMGNELAP
YPNAAKLLNGQMGTFRPLPAKHPNVVRIQTAFIDSLKVLPDAIERYPDAL
HTARWYESIASEPKTMYVVMRRYRQTLHEYVWTRHRNYWTGRVIIAQLLE
ACTYLHKHKVAQRDMKSDNILLEYDFDDEIPQLVVAAFGCALACDNWQVD
YESDEVSLGGNAKTKAPEIATAVPGKNVKVNFEMADTWAAGGLSYEVLTR
SNPFYKLLDTATYQESELPALPSRVNFVARDVIFDLLKRDPNERVKPNIA
ANALNLSLFRMGEDVKQMMEKCGISQMTTLLAGSSKVLSQKINSRLDKVM
NLITAETIMANLAPHLISRAERQLRATFLSRMNREDIWRSLQYFFPAGVQ
LDTPATSSDCLETISSLMSSFSNDSENYEKQQKPAKNGYNNVPLLLRNVI
RTDADGINGIVHRVRSK*

Vector Type

Bacterial

Antibiotic

Amp

100042 R 1000 1001 1047 19007