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Plasmid
pET6HIS SUMO Pink1 trichoplax 162-558 D358A (Codon optimized)
Parent Plasmid
pET15b 6His SUMO1
Expressed
His-SUMO-Trichoplax PINK1 162-558 D358A
DU Number
DU43826
Genbank
XP_002109370.1
Tag
SUMO
Species
Trichoplax adhaerens
Sequence of Insert
View Sequence
GGAGACACCACGAATTCATATAAAGTCGGCCCGCGTCTGGGTTCTCGCAG
TTGCTGTTCTGCGGTGTACGCCACCAAATGCAGTTGTGGCAAAGAATACG
CGATGAAAATGATGTTCAACTACGGTAACTCTAAAGATGACTTCATCCGC
AAAAACTTCCTGCGTGAATATTGCCTGCTGAGTCCGCTGCCGGTGACGCG
TGTTGATGAAATGGAAAGCTCTAAAAACTCCCAGTCACGCATTCTGGTGC
ACCCGAACATCATCCGTCTGGATAAAGTTATCATCGACGAAATCCAATAC
TGTGATGACGCAATGCATGAATACCCGGATGCTCTGCCGCCGTCGCCGCT
GAATCCGAGCGGTCTGGGTCGTCACCGTACCATGTTCCTGTTCATGAAAA
AATACGACATGACGCTGCGCGAATACGTTCATTCCAAAGGCATTATCCCG
AACGATGAAGCAACCATGCTGCTGCTGCAGCTGCTGCAGGGTGTGGACTA
TCTGAACAAAAATTACATTGCTCACCGTGATCTGAAAAGCGACAATATTC
TGATCGAAGAAGTTGATCCGTATCCGCTGCTGGCGATCACCGcCTTCGGC
GAATGCCTGGATGACGCAGCTCTGAAACTGGTTGTCCCGTACATTAGCAA
CTGTATCGATCGCGGCGGTAATAGTTCCCTGATGGCACCGGAAATTAAAA
ACGCTGAACCGGGTCATAATTCTGTTCTGGATTATCGTCGCAGTGACGTC
TGGGCGATTGGCTGCCTGGCCTTTGAAATCTTCGGTCAGGAAAACCCGTT
TCTGAATACCTTCGATTCATCGAGCTATCAAGAATACGAACTGCCGGAAC
TGCCGTCTCGTGTCCCGCTGATTGTGCGTAAAATCGTTCGCAACCTGCTG
CGTAAAGATTCTAGTAATCGTCTGCCGGCATCCCTGGCTATTGCAGCACT
GTCATTTTGGCGTTGGGGTCCGATCAATTGGCGTGCACTGATGATGTATG
AAACCGGTCTGAGTAACCTGAATGAAGAAGATATTCAGCGTTGGCTGTCG
GAAACGGCCTTCCAGCTGAGCATTCAAATCATGCAGTGTCAAGCGCATGG
TCACAAACTGACCCCGGAACTGCAGGCCAAATACCATTTTCTGGTCTCAA
CGAGTGCCGCTGACATCAAACTGGCTGTGGAATCCCTGTATTGCTAAGCG
GCCGC
TTGCTGTTCTGCGGTGTACGCCACCAAATGCAGTTGTGGCAAAGAATACG
CGATGAAAATGATGTTCAACTACGGTAACTCTAAAGATGACTTCATCCGC
AAAAACTTCCTGCGTGAATATTGCCTGCTGAGTCCGCTGCCGGTGACGCG
TGTTGATGAAATGGAAAGCTCTAAAAACTCCCAGTCACGCATTCTGGTGC
ACCCGAACATCATCCGTCTGGATAAAGTTATCATCGACGAAATCCAATAC
TGTGATGACGCAATGCATGAATACCCGGATGCTCTGCCGCCGTCGCCGCT
GAATCCGAGCGGTCTGGGTCGTCACCGTACCATGTTCCTGTTCATGAAAA
AATACGACATGACGCTGCGCGAATACGTTCATTCCAAAGGCATTATCCCG
AACGATGAAGCAACCATGCTGCTGCTGCAGCTGCTGCAGGGTGTGGACTA
TCTGAACAAAAATTACATTGCTCACCGTGATCTGAAAAGCGACAATATTC
TGATCGAAGAAGTTGATCCGTATCCGCTGCTGGCGATCACCGcCTTCGGC
GAATGCCTGGATGACGCAGCTCTGAAACTGGTTGTCCCGTACATTAGCAA
CTGTATCGATCGCGGCGGTAATAGTTCCCTGATGGCACCGGAAATTAAAA
ACGCTGAACCGGGTCATAATTCTGTTCTGGATTATCGTCGCAGTGACGTC
TGGGCGATTGGCTGCCTGGCCTTTGAAATCTTCGGTCAGGAAAACCCGTT
TCTGAATACCTTCGATTCATCGAGCTATCAAGAATACGAACTGCCGGAAC
TGCCGTCTCGTGTCCCGCTGATTGTGCGTAAAATCGTTCGCAACCTGCTG
CGTAAAGATTCTAGTAATCGTCTGCCGGCATCCCTGGCTATTGCAGCACT
GTCATTTTGGCGTTGGGGTCCGATCAATTGGCGTGCACTGATGATGTATG
AAACCGGTCTGAGTAACCTGAATGAAGAAGATATTCAGCGTTGGCTGTCG
GAAACGGCCTTCCAGCTGAGCATTCAAATCATGCAGTGTCAAGCGCATGG
TCACAAACTGACCCCGGAACTGCAGGCCAAATACCATTTTCTGGTCTCAA
CGAGTGCCGCTGACATCAAACTGGCTGTGGAATCCCTGTATTGCTAAGCG
GCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGDTTNSYKVGPRLGSRSCCSAVYATKCSCGKEYAMKMMFNYGNSKDD
FIRKNFLREYCLLSPLPVTRVDEMESSKNSQSRILVHPNIIRLDKVIIDE
IQYCDDAMHEYPDALPPSPLNPSGLGRHRTMFLFMKKYDMTLREYVHSKG
IIPNDEATMLLLQLLQGVDYLNKNYIAHRDLKSDNILIEEVDPYPLLAIT
AFGECLDDAALKLVVPYISNCIDRGGNSSLMAPEIKNAEPGHNSVLDYRR
SDVWAIGCLAFEIFGQENPFLNTFDSSSYQEYELPELPSRVPLIVRKIVR
NLLRKDSSNRLPASLAIAALSFWRWGPINWRALMMYETGLSNLNEEDIQR
WLSETAFQLSIQIMQCQAHGHKLTPELQAKYHFLVSTSAADIKLAVESLY
C*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGDTTNSYKVGPRLGSRSCCSAVYATKCSCGKEYAMKMMFNYGNSKDD
FIRKNFLREYCLLSPLPVTRVDEMESSKNSQSRILVHPNIIRLDKVIIDE
IQYCDDAMHEYPDALPPSPLNPSGLGRHRTMFLFMKKYDMTLREYVHSKG
IIPNDEATMLLLQLLQGVDYLNKNYIAHRDLKSDNILIEEVDPYPLLAIT
AFGECLDDAALKLVVPYISNCIDRGGNSSLMAPEIKNAEPGHNSVLDYRR
SDVWAIGCLAFEIFGQENPFLNTFDSSSYQEYELPELPSRVPLIVRKIVR
NLLRKDSSNRLPASLAIAALSFWRWGPINWRALMMYETGLSNLNEEDIQR
WLSETAFQLSIQIMQCQAHGHKLTPELQAKYHFLVSTSAADIKLAVESLY
C*
Vector Type
Bacterial
Antibiotic
Amp
Comments
in pET vector
100042 R 1000 1001 1047 19007
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