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Plasmid
pET6HIS SUMO Pink1 (mouse) 155-580
Parent Plasmid
pET15b 6His SUMO1
Expressed
His-SUMO-Mouse-PINK1 (155-513)
DU Number
DU43778
Genbank
Q99MQ3
Tag
6His
SUMO
Species
Mouse
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGDYLIGQAIGKGCNAAVYEATMPTLPQHLEKAKHLGLIGKGPDVVLK
GADGEQAPGTPTFPFAIKMMWNISAGSSSEAILSKMSQELVPASRVALAG
EYGAVTYRRSRDGPKQLAPHPNIIRVFRAFTSSVPLLPGALADYPDMLPP
HYYPEGLGHGRTLFLVMKNYPCTLRQYLEEQTPSSRLATMMTLQLLEGVD
HLVQQGIAHRDLKSDNILVEWDSDGCPWLVISDFGCCLADQHVGLRLPFN
SSSVERGGNGSLMAPEVSTAHSGPSAVIDYSKADTWAVGAIAYEIFGLAN
PFYGQGSAHLESRSYQEAQLPEMPESVPPEARRLVRSLLQREASKRPSAR
LAANVLHLSLWGEHLLALKNLKLDKMIAWLLQQSAATLLADRLREKSCVE
TKLQMLFLANLECEALCQAALLLSSWRAAP*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGDYLIGQAIGKGCNAAVYEATMPTLPQHLEKAKHLGLIGKGPDVVLK
GADGEQAPGTPTFPFAIKMMWNISAGSSSEAILSKMSQELVPASRVALAG
EYGAVTYRRSRDGPKQLAPHPNIIRVFRAFTSSVPLLPGALADYPDMLPP
HYYPEGLGHGRTLFLVMKNYPCTLRQYLEEQTPSSRLATMMTLQLLEGVD
HLVQQGIAHRDLKSDNILVEWDSDGCPWLVISDFGCCLADQHVGLRLPFN
SSSVERGGNGSLMAPEVSTAHSGPSAVIDYSKADTWAVGAIAYEIFGLAN
PFYGQGSAHLESRSYQEAQLPEMPESVPPEARRLVRSLLQREASKRPSAR
LAANVLHLSLWGEHLLALKNLKLDKMIAWLLQQSAATLLADRLREKSCVE
TKLQMLFLANLECEALCQAALLLSSWRAAP*
Vector Type
Bacterial
Antibiotic
Amp
Comments
in pET vector as done previously with no overhanging residues between SUMO and start of protein sequence
100042 R 1000 1001 1047 19007
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