Pink1 | LRRK2 Resource

Plasmid

pcDNA5FRT TO Pink1 FLAG T185E T257E

Parent Plasmid

pcDNA5 FRT/TO

Expressed

Pink1 FLAG T185E T257E

DU Number

DU43715

Genbank

Q9BXM7

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

GGATCCGCCACCATGGCGGTGCGACAGGCGCTGGGCCGCGGCCTGCAGCT
GGGTCGAGCGCTGCTGCTGCGCTTCACGGGCAAGCCCGGCCGGGCCTACG
GCTTGGGGCGGCCGGGCCCGGCGGCGGGCTGTGTCCGCGGGGAGCGTCCA
GGCTGGGCCGCAGGACCGGGCGCGGAGCCTCGCAGGGTCGGGCTCGGGCT
CCCTAACCGTCTCCGCTTCTTCCGCCAGTCGGTGGCCGGGCTGGCGGCGC
GGTTGCAGCGGCAGTTCGTGGTGCGGGCCTGGGGCTGCGCGGGCCCTTGC
GGCCGGGCAGTCTTTCTGGCCTTCGGGCTAGGGCTGGGCCTCATCGAGGA
AAAACAGGCGGAGAGCCGGCGGGCGGTCTCGGCCTGTCAGGAGATCCAGG
CAATTTTTACCCAGAAAAGCAAGCCGGGGCCTGACCCGTTGGACACGAGA
CGCTTGCAGGGCTTTCGGCTGGAGGAGTATCTGATAGGGCAGTCCATTGG
TAAGGGCTGCAGTGCTGCTGTGTATGAAGCCACCATGCCTACATTGCCCC
AGAACCTGGAGGTGGAAAAGAGCACCGGGTTGCTTCCAGGGAGAGGCCCA
GGTACCAGTGCACCAGGAGAAGGGCAGGAGCGAGCTCCGGGGGCCCCTGC
CTTCCCCTTGGCCATCAAGATGATGTGGAACATCTCGGCAGGTTCCTCCA
GCGAAGCCATCTTGAACACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGA
GTGGCCTTGGCTGGGGAGTATGGAGCAGTCGAATACAGAAAATCCAAGAG
AGGTCCCAAGCAACTAGCCCCTCACCCCAACATCATCCGGGTTCTCCGCG
CCTTCACCTCTTCCGTGCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCT
GATGTGCTGCCCTCACGCCTCCACCCTGAAGGCCTGGGCCATGGCCGGAC
GCTGTTCCTCGTTATGAAGAACTATCCCTGTACCCTGCGCCAGTACCTTT
GTGTGAACACACCCAGCCCCCGCCTCGCCGCCATGATGCTGCTGCAGCTG
CTGGAAGGCGTGGACCATCTGGTTCAACAGGGCATCGCGCACAGAGACCT
GAAATCCGACAACATCCTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGC
TGGTGATCGCAGATTTTGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTG
CAGTTGCCCTTCAGCAGCTGGTACGTGGATCGGGGCGGAAACGGCTGTCT
GATGGCCCCAGAGGTGTCCACGGCCCGTCCTGGCCCCAGGGCAGTGATTG
ACTACAGCAAGGCTGATGCCTGGGCAGTGGGAGCCATCGCCTATGAAATC
TTCGGGCTTGTCAATCCCTTCTACGGCCAGGGCAAGGCCCACCTTGAAAG
CCGCAGCTACCAAGAGGCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTC
CAGACGTGAGACAGTTGGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAG
AGACCATCTGCCCGAGTAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGG
TGAACATATTCTAGCCCTGAAGAATCTGAAGTTAGACAAGATGGTTGGCT
GGCTCCTCCAACAATCGGCCGCCACTTTGTTGGCCAACAGGCTCACAGAG
AAGTGTTGTGTGGAAACAAAAATGAAGATGCTCTTTCTGGCTAACCTGGA
GTGTGAAACGCTCTGCCAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAG
CCCTGGACTACAAGGACGACGATGACAAGTAGCGGCCGC

Amino Acid Sequence

View Sequence

MAVRQALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGCVRGERPGWAA
GPGAEPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAV
FLAFGLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQG
FRLEEYLIGQSIGKGCSAAVYEATMPTLPQNLEVEKSTGLLPGRGPGTSA
PGEGQERAPGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALA
GEYGAVEYRKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLP
SRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGV
DHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPF
SSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLV
NPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSA
RVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCV
ETKMKMLFLANLECETLCQAALLLCSWRAALDYKDDDDK*

Vector Type

Mammalian

Antibiotic

Amp

Comments

in pcDNA5frtTO vector

100042 R 1000 1001 1047 19007