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Plasmid
pET6HIS SUMO Pink1 Tribolium 154-570 D359A
Parent Plasmid
pET15b 6His SUMO1
Expressed
6HIS SUMO Pink1 Tribolium 154-570 D359A
DU Number
DU43670
Genbank
XP_968367
Tag
6His
SUMO
Species
Tribolium castaneum
Sequence of Insert
View Sequence
TCTAACCCGATCACCCTGAATGATCTGAGTCTGGGTAAACCGATCGCCAA
AGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAGATGACGAAACCG
ATGACAATAAATATCCGTTTGCCCTGAAAATGATGTTCAACTACGATATT
CAGAGCAATTCTATGGAAATCCTGAAAGCTATGTATCGTGAAACCGTTCC
GGCGCGCATGTATTACTCAAACCACGACCTGAACAATTGGGAAATTGAAC
TGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAATATTGTGGCAATC
TTTAGCGTTTTCACCGATCTGATCCAAGAACTGGAAGGTTCTAAAGACCT
GTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAGGTGAAGGTCGCA
ACATGAGCCTGTTTCTGCTGATGAAACGTTATGATTGTAATCTGCAGTCA
TTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCTGCTGCTGGCCCA
ACTGCTGGAAGGCGTGGCGCATATGACGGCCCACGGTATTGCACATCGTG
ATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAACCGGAATCGCCG
ATTCTGGTTATCTCGGcTTTTGGCTGCTGTCTGGCGGACAAAACCAACGG
TCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAGGCGGTAATACGG
CGCTGATGGCCCCGGAAATTATCTGCCAGAAACCGGGCACCTTTAGTGTC
CTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGCAATCGCTTACGA
AATCTTCAACTGTCACAATCCGTTCTACGGCCCGTCTCGCCTGAAAAACT
TCAACTACAAAGAAGGTGATCTGCCGAAACTGCCGGACGAAGTGCCGACC
GTTATTCAGGCACTGGTGGCTAACCTGCTGAAACGTAACCCGAATAAACG
CCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGTTTCTGTGGGCAC
CGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCGACGTCAGGTGAAATT
CTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTGCGAAGGCAAAAT
CAACAACAAATCCTTCGGTGAAAAATTCACCCGTAATTGGCGTCGCACGT
ATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGCGCGAAACTGGCT
AATGTTCGTAATGCTCTGCACTGGATTCAAGAAAACCTGCCGGAACTGGA
TTAAGCGGCCGC
AGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCAAAGATGACGAAACCG
ATGACAATAAATATCCGTTTGCCCTGAAAATGATGTTCAACTACGATATT
CAGAGCAATTCTATGGAAATCCTGAAAGCTATGTATCGTGAAACCGTTCC
GGCGCGCATGTATTACTCAAACCACGACCTGAACAATTGGGAAATTGAAC
TGGCTAACCGTCGCAAACATCTGCCGCCGCACCCGAATATTGTGGCAATC
TTTAGCGTTTTCACCGATCTGATCCAAGAACTGGAAGGTTCTAAAGACCT
GTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGGAAGGTGAAGGTCGCA
ACATGAGCCTGTTTCTGCTGATGAAACGTTATGATTGTAATCTGCAGTCA
TTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCTGCTGCTGCTGGCCCA
ACTGCTGGAAGGCGTGGCGCATATGACGGCCCACGGTATTGCACATCGTG
ATCTGAAATCAGACAACCTGCTGCTGGATACCTCAGAACCGGAATCGCCG
ATTCTGGTTATCTCGGcTTTTGGCTGCTGTCTGGCGGACAAAACCAACGG
TCTGAGCCTGCCGTATACGTCTTACGAAATGGATAAAGGCGGTAATACGG
CGCTGATGGCCCCGGAAATTATCTGCCAGAAACCGGGCACCTTTAGTGTC
CTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGGTGCAATCGCTTACGA
AATCTTCAACTGTCACAATCCGTTCTACGGCCCGTCTCGCCTGAAAAACT
TCAACTACAAAGAAGGTGATCTGCCGAAACTGCCGGACGAAGTGCCGACC
GTTATTCAGGCACTGGTGGCTAACCTGCTGAAACGTAACCCGAATAAACG
CCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGCTGTTTCTGTGGGCAC
CGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCGACGTCAGGTGAAATT
CTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCTGTGCGAAGGCAAAAT
CAACAACAAATCCTTCGGTGAAAAATTCACCCGTAATTGGCGTCGCACGT
ATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGTCGCGCGAAACTGGCT
AATGTTCGTAATGCTCTGCACTGGATTCAAGAAAACCTGCCGGAACTGGA
TTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALKMMF
NYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLPPHPN
IVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMKRYDC
NLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLDTSE
PESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIICQKPG
TFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPKLPD
EVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGLKVPT
SGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLISSFLCR
AKLANVRNALHWIQENLPELD*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGSNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFALKMMF
NYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHLPPHPN
IVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLMKRYDC
NLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLLLDTSE
PESPILVISAFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEIICQKPG
TFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDLPKLPD
EVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPGLKVPT
SGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLISSFLCR
AKLANVRNALHWIQENLPELD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
in pET - please make wild-type and kinase-inactive versions (D359A)
100042 R 1000 1001 1047 19007
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