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Plasmid
pET6HIS SUMO Pink1 Tribolium 149-570
Parent Plasmid
pET15b 6His SUMO1
Expressed
6His-SUMO-TcPINK1 149-570
DU Number
DU43659
Genbank
XP_968367
Tag
6His
SUMO
Species
Tribolium castaneum
Sequence of Insert
View Sequence
GAAAGCCGCTTTGAATCTAACCCGATCACCCTGAATGATCTGAGTCTGGG
TAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCA
AAGATGACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATG
TTCAACTACGATATTCAGAGCAATTCTATGGAAATCCTGAAAGCTATGTA
TCGTGAAACCGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACA
ATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCG
AATATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGA
AGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGG
AAGGTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGAT
TGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCT
GCTGCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACG
GTATTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCA
GAACCGGAATCGCCGATTCTGGTTATCTCGGATTTTGGCTGCTGTCTGGC
GGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATA
AAGGCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCG
GGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGG
TGCAATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGT
CTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTGCCGAAACTGCCG
GACGAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAACG
TAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGC
TGTTTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCG
ACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCT
GTGCGAAGGCAAAATCAACAACAAATCCTTCGGTGAAAAATTCACCCGTA
ATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGT
CGCGCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAA
CCTGCCGGAACTGGATTAAGCGGCCGC
TAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAAAAGTCA
AAGATGACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAAATGATG
TTCAACTACGATATTCAGAGCAATTCTATGGAAATCCTGAAAGCTATGTA
TCGTGAAACCGTTCCGGCGCGCATGTATTACTCAAACCACGACCTGAACA
ATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCGCACCCG
AATATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGAACTGGA
AGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGCATCCGG
AAGGTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGTTATGAT
TGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCACCTCCCT
GCTGCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGGCCCACG
GTATTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTGGATACCTCA
GAACCGGAATCGCCGATTCTGGTTATCTCGGATTTTGGCTGCTGTCTGGC
GGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAATGGATA
AAGGCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAGAAACCG
GGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGCGGTGGG
TGCAATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACGGCCCGT
CTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTGCCGAAACTGCCG
GACGAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCTGAAACG
TAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCTGCCAGC
TGTTTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAAGTTCCG
ACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAAAGTCCT
GTGCGAAGGCAAAATCAACAACAAATCCTTCGGTGAAAAATTCACCCGTA
ATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTCCTGTGT
CGCGCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCAAGAAAA
CCTGCCGGAACTGGATTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGESRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFA
LKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHL
PPHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLM
KRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLL
LDTSEPESPILVISDFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEII
CQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDL
PKLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPG
LKVPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLIS
SFLCRAKLANVRNALHWIQENLPELD*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGESRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYPFA
LKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRKHL
PPHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFLLM
KRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDNLL
LDTSEPESPILVISDFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPEII
CQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEGDL
PKLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLKPG
LKVPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLLIS
SFLCRAKLANVRNALHWIQENLPELD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
in pET - please make wild-type and kinase-inactive versions (D359A)
100042 R 1000 1001 1047 19007
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