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Plasmid
pET6HIS SUMO Pink1 tribolium 147-570
Parent Plasmid
pET15b 6His SUMO1
Expressed
His-SUMO-TcPINK1 (147-end)
DU Number
DU43658
Genbank
XP_968367
Tag
6His
SUMO
Species
Tribolium castaneum
Sequence of Insert
View Sequence
ATCGACGAAAGCCGCTTTGAATCTAACCCGATCACCCTGAATGATCTGAG
TCTGGGTAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAA
AAGTCAAAGATGACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAA
ATGATGTTCAACTACGATATTCAGAGCAATTCTATGGAAATCCTGAAAGC
TATGTATCGTGAAACCGTTCCGGCGCGCATGTATTACTCAAACCACGACC
TGAACAATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCG
CACCCGAATATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGA
ACTGGAAGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGC
ATCCGGAAGGTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGT
TATGATTGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCAC
CTCCCTGCTGCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGG
CCCACGGTATTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTGGAT
ACCTCAGAACCGGAATCGCCGATTCTGGTTATCTCGGATTTTGGCTGCTG
TCTGGCGGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAA
TGGATAAAGGCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAG
AAACCGGGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGC
GGTGGGTGCAATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACG
GCCCGTCTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTGCCGAAA
CTGCCGGACGAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCT
GAAACGTAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCT
GCCAGCTGTTTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAA
GTTCCGACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAA
AGTCCTGTGCGAAGGCAAAATCAACAACAAATCCTTCGGTGAAAAATTCA
CCCGTAATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTC
CTGTGTCGCGCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCA
AGAAAACCTGCCGGAACTGGATTAAGCGGCCGC
TCTGGGTAAACCGATCGCCAAAGGCACGAACGGTGTGGTTTACTCCGCAA
AAGTCAAAGATGACGAAACCGATGACAATAAATATCCGTTTGCCCTGAAA
ATGATGTTCAACTACGATATTCAGAGCAATTCTATGGAAATCCTGAAAGC
TATGTATCGTGAAACCGTTCCGGCGCGCATGTATTACTCAAACCACGACC
TGAACAATTGGGAAATTGAACTGGCTAACCGTCGCAAACATCTGCCGCCG
CACCCGAATATTGTGGCAATCTTTAGCGTTTTCACCGATCTGATCCAAGA
ACTGGAAGGTTCTAAAGACCTGTATCCGGCAGCGCTGCCGCCGCGTCTGC
ATCCGGAAGGTGAAGGTCGCAACATGAGCCTGTTTCTGCTGATGAAACGT
TATGATTGTAATCTGCAGTCATTCCTGAGCACCGCTCCGAGTACCCGCAC
CTCCCTGCTGCTGCTGGCCCAACTGCTGGAAGGCGTGGCGCATATGACGG
CCCACGGTATTGCACATCGTGATCTGAAATCAGACAACCTGCTGCTGGAT
ACCTCAGAACCGGAATCGCCGATTCTGGTTATCTCGGATTTTGGCTGCTG
TCTGGCGGACAAAACCAACGGTCTGAGCCTGCCGTATACGTCTTACGAAA
TGGATAAAGGCGGTAATACGGCGCTGATGGCCCCGGAAATTATCTGCCAG
AAACCGGGCACCTTTAGTGTCCTGAATTATTCCAAAGCTGACCTGTGGGC
GGTGGGTGCAATCGCTTACGAAATCTTCAACTGTCACAATCCGTTCTACG
GCCCGTCTCGCCTGAAAAACTTCAACTACAAAGAAGGTGATCTGCCGAAA
CTGCCGGACGAAGTGCCGACCGTTATTCAGGCACTGGTGGCTAACCTGCT
GAAACGTAACCCGAATAAACGCCTGGACCCGGAAGTTGCGGCCAATGTCT
GCCAGCTGTTTCTGTGGGCACCGAGTACCTGGCTGAAACCGGGTCTGAAA
GTTCCGACGTCAGGTGAAATTCTGCAATGGCTGCTGTCGCTGACCACGAA
AGTCCTGTGCGAAGGCAAAATCAACAACAAATCCTTCGGTGAAAAATTCA
CCCGTAATTGGCGTCGCACGTATCCGGAATACCTGCTGATTAGTTCCTTC
CTGTGTCGCGCGAAACTGGCTAATGTTCGTAATGCTCTGCACTGGATTCA
AGAAAACCTGCCGGAACTGGATTAAGCGGCCGC
Amino Acid Sequence
View Sequence
MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGIDESRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYP
FALKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRK
HLPPHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFL
LMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDN
LLLDTSEPESPILVISDFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPE
IICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEG
DLPKLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLK
PGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLL
ISSFLCRAKLANVRNALHWIQENLPELD*
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGIDESRFESNPITLNDLSLGKPIAKGTNGVVYSAKVKDDETDDNKYP
FALKMMFNYDIQSNSMEILKAMYRETVPARMYYSNHDLNNWEIELANRRK
HLPPHPNIVAIFSVFTDLIQELEGSKDLYPAALPPRLHPEGEGRNMSLFL
LMKRYDCNLQSFLSTAPSTRTSLLLLAQLLEGVAHMTAHGIAHRDLKSDN
LLLDTSEPESPILVISDFGCCLADKTNGLSLPYTSYEMDKGGNTALMAPE
IICQKPGTFSVLNYSKADLWAVGAIAYEIFNCHNPFYGPSRLKNFNYKEG
DLPKLPDEVPTVIQALVANLLKRNPNKRLDPEVAANVCQLFLWAPSTWLK
PGLKVPTSGEILQWLLSLTTKVLCEGKINNKSFGEKFTRNWRRTYPEYLL
ISSFLCRAKLANVRNALHWIQENLPELD*
Vector Type
Bacterial
Antibiotic
Amp
Comments
in pET - please make wild-type and kinase-inactive versions (D359A)
100042 R 1000 1001 1047 19007
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