PINK1 | LRRK2 Resource

Synonyms

BRPK, FLJ27236, PARK6

Plasmid

pcDNA5frtTO HSP60 1-27 + PINK1 150-581 + C-FLAG

Parent Plasmid

pcDNA5 FRT/TO

Expressed

HSP60 (1-27) - PINK1 (150-581) -FLAG

DU Number

DU44362

Genbank

NM_032409.2

Tag

FLAG

Species

Human

Sequence of Insert

View Sequence

AAGCTTGCCACCATGCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACC
GGTGTCCAGGGTACTGGCTCCTCATCTCACTCGGGCTTATGCCGGATCCA
TGGGCTTTCGGCTGGAGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGC
TGCAGTGCTGCTGTGTATGAAGCCACCATGCCTACATTGCCCCAGAACCT
GGAGGTGACAAAGAGCACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCA
GTGCACCAGGAGAAGGGCAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCC
TTGGCCATCAAGATGATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGC
CATCTTGAACACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCT
TGGCTGGGGAGTATGGAGCAGTCACTTACAGAAAATCCAAGAGAGGTCCC
AAGCAACTAGCCCCTCACCCCAACATCATCCGGGTTCTCCGCGCCTTCAC
CTCTTCCGTGCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGC
TGCCCTCACGCCTCCACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTC
CTCGTTATGAAGAACTATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAA
CACACCCAGCCCCCGCCTCGCCGCCATGATGCTGCTGCAGCTGCTGGAAG
GCGTGGACCATCTGGTTCAACAGGGCATCGCGCACAGAGACCTGAAATCC
GACAACATCCTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGCTGGTGAT
CGCAGATTTTGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGC
CCTTCAGCAGCTGGTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCC
CCAGAGGTGTCCACGGCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAG
CAAGGCTGATGCCTGGGCAGTGGGAGCCATCGCCTATGAAATCTTCGGGC
TTGTCAATCCCTTCTACGGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGC
TACCAAGAGGCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTCCAGACGT
GAGACAGTTGGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCAT
CTGCCCGAGTAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGGTGAACAT
ATTCTAGCCCTGAAGAATCTGAAGTTAGACAAGATGGTTGGCTGGCTCCT
CCAACAATCGGCCGCCACTTTGTTGGCCAACAGGCTCACAGAGAAGTGTT
GTGTGGAAACAAAAATGAAGATGCTCTTTCTGGCTAACCTGGAGTGTGAA
ACGCTCTGCCAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAGCCCTGGA
CTACAAGGACGATGACGATAAGTGAGCGGCCGC

Amino Acid Sequence

View Sequence

MLRLPTVFRQMRPVSRVLAPHLTRAYAGSMGFRLEEYLIGQSIGKGCSAA
VYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIK
MMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLA
PHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMK
NYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNIL
VELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVS
TARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEA
QLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILAL
KNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQ
AALLLCSWRAALDYKDDDDK*

Vector Type

Mammalian

Antibiotic

Amp

Comments

HSP60 1-27 at N-terminus and C-FLAG. HSP60 1-27 may be swapped HindIII/BamHI and the PINK1-FLAG using BamHI/NotI.

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