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Synonyms
BRPK, FLJ27236, PARK6
Plasmid
pcDNA5frtTO HSP60 1-27 + PINK1 150-513 + C-FLAG
Parent Plasmid
pcDNA5 FRT/TO
Expressed
HSP60 (1-27) - PINK1 (150-513) -FLAG
DU Number
DU44360
Genbank
NM_032409.2
Tag
FLAG
Species
Human
Sequence of Insert
View Sequence
AAGCTTGCCACCATGCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACC
GGTGTCCAGGGTACTGGCTCCTCATCTCACTCGGGCTTATGCCGGATCCA
TGGGCTTTCGGCTGGAGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGC
TGCAGTGCTGCTGTGTATGAAGCCACCATGCCTACATTGCCCCAGAACCT
GGAGGTGACAAAGAGCACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCA
GTGCACCAGGAGAAGGGCAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCC
TTGGCCATCAAGATGATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGC
CATCTTGAACACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCT
TGGCTGGGGAGTATGGAGCAGTCACTTACAGAAAATCCAAGAGAGGTCCC
AAGCAACTAGCCCCTCACCCCAACATCATCCGGGTTCTCCGCGCCTTCAC
CTCTTCCGTGCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGC
TGCCCTCACGCCTCCACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTC
CTCGTTATGAAGAACTATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAA
CACACCCAGCCCCCGCCTCGCCGCCATGATGCTGCTGCAGCTGCTGGAAG
GCGTGGACCATCTGGTTCAACAGGGCATCGCGCACAGAGACCTGAAATCC
GACAACATCCTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGCTGGTGAT
CGCAGATTTTGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGC
CCTTCAGCAGCTGGTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCC
CCAGAGGTGTCCACGGCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAG
CAAGGCTGATGCCTGGGCAGTGGGAGCCATCGCCTATGAAATCTTCGGGC
TTGTCAATCCCTTCTACGGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGC
TACCAAGAGGCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTCCAGACGT
GAGACAGTTGGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCAT
CTGCCCGAGTAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGGTGACTAC
AAGGACGATGACGATAAGTGAGCGGCCGC
Amino Acid Sequence
View Sequence
MLRLPTVFRQMRPVSRVLAPHLTRAYAGSMGFRLEEYLIGQSIGKGCSAA
VYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIK
MMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLA
PHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMK
NYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNIL
VELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVS
TARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEA
QLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGDYKDDD
DK*
VYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIK
MMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLA
PHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMK
NYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNIL
VELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVS
TARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEA
QLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGDYKDDD
DK*
Vector Type
Mammalian
Antibiotic
Amp
Comments
HSP60 1-27 at N-terminus and C-FLAG. HSP60 1-27 may be swapped HindIII/BamHI and the PINK1-FLAG using BamHI/NotI.
100042 R 1000 1001 1047 19007
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