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Synonyms
BRPK, FLJ27236, PARK6
Plasmid
pMal4c PINK1d D501A
Parent Plasmid
pMAL4C
Expressed
MBP-PINK1d D501A
DU Number
DU37791
Genbank
NM_
Tag
MBP
Species
Drosophila Melanogaster
Sequence of Insert
View Sequence
GGATCCATGTCTGTGAGACTGCTGACCGTGCGACTGATCAAACATGGTCGCTACATTTTGCGCAGCTATTGTAAACGTGATATACACGCCAACATTTTGGACCAGAATCAATTGAAGACAAGAAGCAAGCGAGGCTTTCCCCTACCCTCCACCGCTGCCAATGTGCTGCGGACAACGCCCCAGCAGGCGGCCAAATCGGTGGTCAATGTAGTGCCCCGCACCATCAACTCCCCGTCGGGATCACCGTTTAATGGCAGCGGCAGTAGCCCCACCAGCAGCAGTGGAATCTTCCGAGTGGGCCAGCATGCGCGCAAATTGTTCATCGACAACATCCTCAGCCGGGTGACCACCACCTACTCGGAGGATCTGCGCCAGCGCGCTACCCGCAAGCTATTCTTTGGCGATTCAGCGCCCTTCTTCGCCCTGATTGGCGTTAGTCTGGCCTCCGGATCGGGTGTGCTCAGCAAGGAGGATGAACTGGAGGGCGTGTGCTGGGAGATTCGGGAGGCAGCTAGCCGGCTGCAGAACGCCTGGAATCACGACGAGATCTCCGATACGCTAGACAGCAAGTTCACCATCGATGACTTGGAAATCGGTCCGCCCATAGCCAAAGGTTGTGCCGCTGTCGTCTATGCAGCGGATTTCAAGAAAGATGTTGCCTCGGATGGTGCATCCCTGCATACCGATGCGCAGCCGCAGGCAACGCCAGCCTTTGCGCCGAATAGCTGGAGTACCCACGAGATGATGTCGCCGCTGCAGAACATGTCGCGCTTTGTTCACAACTTTGGCGGCTCTGTGGACAACGTCTTCCACTACAGCCAGCCATCTGCGGCCAGTGATTTCGTGGGCGCCCAGTCGAGGGAACAGGACCAGCGGCACCACGAGCAACAGCAGCATCAGAATCAGGAACAAGAGCAGCATCAGAACCAGGAGCCCAGCAGCAGTGCCTTCAATGTGACTTCTCCAGCGAATTCAAACATCAACAGCTCTGTGGACAGTTATCCACTGGCACTCAAAATGATGTTCAACTACGACATCCAGAGCAACGCTCTGTCCATACTGCGTGCCATGTACAAGGAGACGGTACCGGCACGCCAGCGCGGCATGAATGAGGCCGCCGATGAGTGGGAACGTTTGCTGCAGAATCAAACTGTTCACCTGCCGCGGCATCCGAACATTGTCTGTATGTTTGGCTTCTTTTGCGACGAGGTGCGCAACTTTCCCGATGGCCATCTCCTGTATCCGGTGGCGCAGCCGCAACGCATCAATCCACAGGGCTATGGCCGCAATATGTCGCTGTATCTGCTGATGAAACGCTACGATCACAGTCTTCGCGGCCTGCTCGATAGCCAGGATCTGAGCACGCGCAATCGCATCCTGCTGCTGGCTCAAATGCTCGAGGCTGTCAACCATTTGAGCCGTCACGGCGTTGCCCATCGTGACCTAAAGTCTGATAATGTGCTAATCGAGCTGCAGGACGATGCGGCGCCGGTGCTAGTGCTCTCCGCCTTTGGCTGTTGTCTGGCGGACAAGGTGCATGGCCTGCGCCTGCCGTACGTTTCGCACGACGTCGACAAGGGCGGCAATGCGGCGTTGATGGCGCCAGAGATCTTCAATACGATGCCCGGTCCGTTTGCCGTACTCAATTATGGCAAGGCCGATCTGTGGGCTTGCGGTGCCCTGGCTTACGAAATCTTTGGCAACCGCAATCCGTTCTATTCGAGCAGCGGTGGGATGGCGCGCGAACGCGGTGAGATGACGCTTTCGCTGAGGAACAGCGATTACCGACAGGACCAATTGCCGCCAATGAGCGATGCTTGCCCGCCGCTGCTGCAACAGCTGGTCTACAACATCCTCAATCCCAACCCGTCCAAGCGGGTCAGTCCGGATATTGCGGCAAATGTGGTGCAGCTGTTCCTCTGGGCCCCATCCAATTGGCTTAAGGCGGGCGGCATGCCCAACAGTCCGGAGATCCTACAGTGGCTCCTTTCGCTGACCACTAAGATTATGTGCGAGGGTCGTCCACAGATGGGCGCCGGATTGATGCCAGTGGCCAGCTGTGGCAATAGGCGCGCCTATGTGGAGTACCTGCTCATATGCAGCTTTCTGGCACGCGCCCGGCTGCGTCGCATTCGCGGCGCCCTCAACTGGATACAGAATGTGGTGGCGTAGGCGGCCGC
Amino Acid Sequence
View Sequence
MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGDDDDKVPEFLEVLFQGPGSMSVRLLTVRLIKHGRYILRSYCKRDIHANILDQNQLKTRSKRGFPLPSTAANVLRTTPQQAAKSVVNVVPRTINSPSGSPFNGSGSSPTSSSGIFRVGQHARKLFIDNILSRVTTTYSEDLRQRATRKLFFGDSAPFFALIGVSLASGSGVLSKEDELEGVCWEIREAASRLQNAWNHDEISDTLDSKFTIDDLEIGPPIAKGCAAVVYAADFKKDVASDGASLHTDAQPQATPAFAPNSWSTHEMMSPLQNMSRFVHNFGGSVDNVFHYSQPSAASDFVGAQSREQDQRHHEQQQHQNQEQEQHQNQEPSSSAFNVTSPANSNINSSVDSYPLALKMMFNYDIQSNALSILRAMYKETVPARQRGMNEAADEWERLLQNQTVHLPRHPNIVCMFGFFCDEVRNFPDGHLLYPVAQPQRINPQGYGRNMSLYLLMKRYDHSLRGLLDSQDLSTRNRILLLAQMLEAVNHLSRHGVAHRDLKSDNVLIELQDDAAPVLVLSAFGCCLADKVHGLRLPYVSHDVDKGGNAALMAPEIFNTMPGPFAVLNYGKADLWACGALAYEIFGNRNPFYSSSGGMARERGEMTLSLRNSDYRQDQLPPMSDACPPLLQQLVYNILNPNPSKRVSPDIAANVVQLFLWAPSNWLKAGGMPNSPEILQWLLSLTTKIMCEGRPQMGAGLMPVASCGNRRAYVEYLLICSFLARARLRRIRGALNWIQNVVA*
Vector Type
Bacterial
Antibiotic
Amp
Comments
Contains T>C silent mutation to KO internal BamHI site. D501A mutation to render kinase dead
100042 R 1000 1001 1047 19007
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