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Plasmid
pEGFP-C1-PINK1 125-581
Parent Plasmid
pEGFPC1
Expressed
GFP-PINK1 125-581
DU Number
DU3635
Genbank
BC028215
Tag
GFP
Species
Human
Sequence of Insert
View Sequence
GGATCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAAAAGCAAGCCGGG
GCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTCGGCTGGAGGAGT
ATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCTGTGTATGAA
GCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGACAAAGAGCACCGG
GTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAGGGCAGG
AGCGAGCTGCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGATGATGTGG
AACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAATGAGCCA
GGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATGGAGCAG
TCACTTACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCCCCTCACCCC
AACATCATCCGGGTTCTCCGCGCCTTCACCTCTTCCGTGCCGCTGCTGCC
AGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCCTCCACCCTG
AAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAACTATCCC
TGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCGCCTCGC
CGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGGTTCAAC
AGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCTTGTGGAGCTG
GACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGATTTTGGCTGCTGCCT
GGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCAGCTGGTACGTGG
ATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCCACGGCCCGT
CCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGCCTGGGCAGT
GGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCTTCTACGGCC
AGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAGGCTCAGCTACCT
GCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTTGGTGAGGGCACT
GCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAGTAGCCGCAAATG
TGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCCCTGAAGAATCTG
AAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATCGGCCGCCACTTT
GTTGGCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAACAAAAATGAAGA
TGCTCTTTCTGGCTAACCTGGAGTGTGAAACGCTCTGCCAGGCAGCCCTC
CTCCTCTGCTCATGGAGGGCAGCCCTGTGAGCGGCCGCGCAAGGGCGAAT
TCTGCAGATATCCATCACACTGGCGGCCGCTCGAG
GCCTGACCCGTTGGACACGAGACGCTTGCAGGGCTTTCGGCTGGAGGAGT
ATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTGCTGCTGTGTATGAA
GCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGACAAAGAGCACCGG
GTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAGGGCAGG
AGCGAGCTGCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGATGATGTGG
AACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAATGAGCCA
GGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATGGAGCAG
TCACTTACAGAAAATCCAAGAGAGGTCCCAAGCAACTAGCCCCTCACCCC
AACATCATCCGGGTTCTCCGCGCCTTCACCTCTTCCGTGCCGCTGCTGCC
AGGGGCCCTGGTCGACTACCCTGATGTGCTGCCCTCACGCCTCCACCCTG
AAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAACTATCCC
TGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCGCCTCGC
CGCCATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGGTTCAAC
AGGGCATCGCGCACAGAGACCTGAAATCCGACAACATCCTTGTGGAGCTG
GACCCAGACGGCTGCCCCTGGCTGGTGATCGCAGATTTTGGCTGCTGCCT
GGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTCAGCAGCTGGTACGTGG
ATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCCACGGCCCGT
CCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGCCTGGGCAGT
GGGAGCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCTTCTACGGCC
AGGGCAAGGCCCACCTTGAAAGCCGCAGCTACCAAGAGGCTCAGCTACCT
GCACTGCCCGAGTCAGTGCCTCCAGACGTGAGACAGTTGGTGAGGGCACT
GCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCCCGAGTAGCCGCAAATG
TGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCCCTGAAGAATCTG
AAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATCGGCCGCCACTTT
GTTGGCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAACAAAAATGAAGA
TGCTCTTTCTGGCTAACCTGGAGTGTGAAACGCTCTGCCAGGCAGCCCTC
CTCCTCTGCTCATGGAGGGCAGCCCTGTGAGCGGCCGCGCAAGGGCGAAT
TCTGCAGATATCCATCACACTGGCGGCCGCTCGAG
Amino Acid Sequence
View Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSCQEIQA
IFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSAAVYEATMPTLPQ
NLEVTKSTGLLPGRGPGTSAPGEGQERAAGAPAFPLAIKMMWNISAGSSS
EAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRA
FTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLC
VNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWL
VIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVID
YSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPP
DVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGW
LLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAA
L*
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSCQEIQA
IFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSAAVYEATMPTLPQ
NLEVTKSTGLLPGRGPGTSAPGEGQERAAGAPAFPLAIKMMWNISAGSSS
EAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRA
FTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLC
VNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWL
VIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVID
YSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPP
DVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGW
LLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAA
L*
Vector Type
Mammalian
Antibiotic
Kan
Comments
N terminal GFP for mammalian expression
100042 R 1000 1001 1047 19007
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