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PINK1

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Plasmid
pEGFP-C1-PINK1 1573-1574 ins
Parent Plasmid
pEGFPC1
Expressed
GFP-PINK1 1573-1574 ins
DU Number
DU3588
Genbank
BC028215
Tag
GFP
Species
Human
Sequence of Insert
GGATCCGCGGTGCGACAGGCGCTGGGCCGCGGCCTGCAGCTGGGTCGAGC
GCTGCTGCTGCGCTTCACGGGCAAGCCCGGCCGGGCCTACGGCTTGGGGC
GGCCGGGCCCGGCGGCGGGCTGTGTCCGCGGGGAGCGTCCAGGCTGGGCC
GCAGGACCGGGCGCGGAGCCTCGCAGGGTCGGGCTCGGGCTCCCTAACCG
TCTCCGCTTCTTCCGCCAGTCGGTGGCCGGGCTGGCGGCGCGGTTGCAGC
GGCAGTTCGTGGTGCGGGCCTGGGGCTGCGCGGGCCCTTGCGGCCGGGCA
GTCTTTCTGGCCTTCGGGCTAGGGCTGGGCCTCATCGAGGAAAAACAGGC
GGAGAGCCGGCGGGCGGTCTCGGCCTGTCAGGAGATCCAGGCAATTTTTA
CCCAGAAAAGCAAGCCGGGGCCTGACCCGTTGGACACGAGACGCTTGCAG
GGCTTTCGGCTGGAGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTG
CAGTGCTGCTGTGTATGAAGCCACCATGCCTACATTGCCCCAGAACCTGG
AGGTGACAAAGAGCACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGT
GCACCAGGAGAAGGGCAGGAGCGAGCTGCGGGGGCCCCTGCCTTCCCCTT
GGCCATCAAGATGATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCA
TCTTGAACACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTG
GCTGGGGAGTATGGAGCAGTCACTTACAGAAAATCCAAGAGAGGTCCCAA
GCAACTAGCCCCTCACCCCAACATCATCCGGGTTCTCCGCGCCTTCACCT
CTTCCGTGCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTG
CCCTCACGCCTCCACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCT
CGTTATGAAGAACTATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACA
CACCCAGCCCCCGCCTCGCCGCCATGATGCTGCTGCAGCTGCTGGAAGGC
GTGGACCATCTGGTTCAACAGGGCATCGCGCACAGAGACCTGAAATCCGA
CAACATCCTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGCTGGTGATCG
CAGATTTTGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCC
TTCAGCAGCTGGTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCC
AGAGGTGTCCACGGCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCA
AGGCTGATGCCTGGGCAGTGGGAGCCATCGCCTATGAAATCTTCGGGCTT
GTCAATCCCTTCTACGGCCAGGGCAAGGCCCACCTTGAAAGCCGCAGCTA
CCAAGAGGCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTCCAGACGTGA
GACAGTTGGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCT
GCCCGAGTAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGGTGAACATAT
TCTAGCCCTGAAGAATCTGAAGTTATTAGGACAAGATGGTTGGCTGGCTC
CTCCAACAATCGGCCGCCACTTTGTTGGCCAACAGGCTCACAGAGAAGTG
TTGTGTGGAAACAAAAATGAAGATGCTCTTTCTGGCTAACCTGGAGTGTG
AAACGCTCTGCCAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAGCCCTG
TGAGCGGCCGCGCAAGGGCGAATTCCAGCACACTGGCGGCCGTTACTAGT
GGATCC
Amino Acid Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSAVRQALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGC
VRGERPGWAAGPGAEPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAW
GCAGPCGRAVFLAFGLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGP
DPLDTRRLQGFRLEEYLIGQSIGKGCSAAVYEATMPTLPQNLEVTKSTGL
LPGRGPGTSAPGEGQERAAGAPAFPLAIKMMWNISAGSSSEAILNTMSQE
LVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPG
ALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAA
MMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLA
DESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVG
AIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALL
QREASKRPSARVAANVLHLSLWGEHILALKNLKLLGQDGWLAPPTIGRHF
VGQQAHREVLCGNKNEDALSG*
Vector Type
Mammalian
Antibiotic
Kan
Comments
N terminal GFP for mammalian expression. Insertion causes frame change after aa 524 leading truncation.
100042 R 1000 1001 1047 19007
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