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Full Name
RAB1A phospho Ser 114
Immuno Sequence
CQEIDRYAS*ENVNKLR [residues 107 - 120 of human]
Mono/Poly clonal
Polyclonal
Sheep No
S817D
Aliquot
0.1mg
100041 R 1000 1001 1047 19007
Gel Image
Gel Description
HeLa Flp-in Trex cells stably expressing Flag-empty, Flag-PINK1 WT (wild-type) or Flag-PINK1 KI (Kinase inactive) were transfected with HA-Rab1B for 24h before Doxycycline (0.1μg/ml) treatment for 24h. All cells were treated with 10μM CCCP for 3h. Whole cell lysates (0.25 mg) were immunoprecipiated with anti-HA agarose and immunoblotted with Rab1A/1B phospho-Ser114/Ser111 antibodies. Binding of the primary antibody was detected using anti-sheep (1:10,000) and visualised with ECL. Note:5th bleed works the best for detecting Rab1B Ser111 phosphorylation. Only Rab1B were test because HA-Rab1A was not expressed well by transient transfection.